Rapid Fluorescent Screening for Bifunctional Amine-Acid Catalysts: Efficient Syntheses of Quaternary Carbon-Containing Aldols under Organocatalysis

Article abstract of DOI:10.1021/ol035651p

(Equation presented) Direct catalytic aldol reactions of α.α-dialkylaldehyde donors and arylaldehyde acceptors have been performed using pyrrolidine-acetic acid bifunctional catalysts. This general and practical amine-acid combination was identified by sc

Full text of DOI:10.1021/ol035651p

ORGANIC
LETTERS
2
003
Vol. 5, No. 23
369-4372
Rapid Fluorescent Screening for
Bifunctional Amine−Acid Catalysts:
Efficient Syntheses of Quaternary
Carbon-Containing Aldols under
Organocatalysis
4
†
Nobuyuki Mase, Fujie Tanaka,* and Carlos F. Barbas, III*
The Skaggs Institute for Chemical Biology and the Departments of Chemistry and
Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road,
La Jolla, California 92037
ftanaka@scripps.edu; carlos@scripps.edu.
Received August 29, 2003
ABSTRACT
Direct catalytic aldol reactions of r,r-dialkylaldehyde donors and arylaldehyde acceptors have been performed using pyrrolidine−acetic acid
bifunctional catalysts. This general and practical amine−acid combination was identified by screening catalysts using a new fluorescent
detection system for carbon−carbon bond formation. Using 0.05 equiv of pyrrolidine and 0.25 equiv of acetic acid as catalyst, we obtained
r,r-dialkylaldol product in 96% yield after 2 h at ambient temperature. Proline was a poor catalyst of this reaction.
The direct aldol reaction is one of the most powerful and
efficient methods for carbon-carbon bond formation. Within
the aldol reaction manifold, the development of standard
syntheses of aldol products possessing a quaternary carbon
carbon atom. Here, we report the intermolecular direct aldol
reaction of R,R-dialkylaldehydes 1 to provide the aldol
products 3 (Scheme 1).
1
has proven to be one of the more challenging topics.
Recently, Shibasaki et al. reported direct aldol reactions of
R-hydroxyketones and aldehydes using an Et Zn/linked-
2
Scheme 1. Direct Aldol Reaction To Form a Quaternary
Carbon Atom via an Enamine Intermediate
BINOL complex for the asymmetric construction of a chiral
quaternary carbon stereocenter.2 Results using organo-
catalysts such as L-proline and small amines have been
3
,4
published; however, there has been no report of a direct
catalytic aldol reaction that generates a tetra-substituted
†
Present address: Department of Molecular Science, Faculty of Engi-
neering, Shizuoka University, 3-5-1 Johoku, Hamamatsu 432-8561, Japan.
(
1) Review: Corey, E. J.; Guzman-Perez, A. Angew. Chem., Int. Ed.
First, we examined whether L-proline was a useful catalyst
for this type of reaction. When the reaction was performed
using isobutyraldehyde and p-nitrobenzaldehyde in the
presence of L-proline according to our typical conditions,3b
formation of the R,R-dimethylaldol product was slow and
1
998, 37, 389-401. Aldol-Tishchenko reaction of naked aldehyde:
Mascarenhas, C. M.; Miller, S. P.; White, P. S.; Morken, J. P. Angew. Chem.,
Int. Ed. 2001, 40, 601-603.
(2) Kumagai, N.; Matsunaga, S.; Kinoshita, T.; Harada, S.; Okada, S.;
Sakamoto, S.; Yamaguchi, K.; Shibasaki, M. J. Am. Chem. Soc. 2003, 125,
169-2178.
2
1
0.1021/ol035651p CCC: $25.00 © 2003 American Chemical Society
Published on Web 10/24/2003

the yield was unacceptably low (3 d, 34% yield). Therefore,
we decided to search for efficient conditions for this
challenging reaction. Since an amine-catalyzed aldol reaction
proceeds via an enamine intermediate 2, acceleration of the
formation of 2 may be a key to enhancing the construction
of the R,R-dialkylaldol product 3. Recently, we have
developed a fluorescent detection system to monitor the
progress of C-C bond formation using the fluorogenic
5
maleimide 4. The acetone enamine generated in situ reacts
with 4 to give 6, which exhibits a high fluorescence
compared to that of 4. Thus, this assay system should be
useful in ranking catalysts of enamine formation. Catalysts
that efficiently form an enamine with acetone should also
be efficient in forming enamines with other carbonyl
compound, in particular aldehydes. Since it is well-known
that pyrrolidine can be used in enamine synthesis and that
6
this process is catalyzed by acids, we have screened
pyrrolidine-acid combinations. The screening was initially
performed by using various acid additives, such as Lewis,
Brønsted, and organic acids on the reaction of 4 with 5 in
the presence of pyrrolidine. Reactions were performed with
pyrrolidine (3 mM), acid (3 mM), and 4 (6 µM) in 20%
acetone/80% DMSO, and the initial reaction velocities were
determined by monitoring the fluorescence (λex 315 nm, λem
Figure 1. Initial velocities of reactions of pyrrolidine-acid
combinations using fluorogenic substrate 4. Conditions: see text.
RFU ) Relative fluorescence unit. Acid additives: 1, none; 2,
365 nm) over 20 min. Results are shown in Figure 1. Eight
Sc(OTf)
Eu(OTf)
D-(+)-10-camphorsulfonic acid; 13, HNO
CH CO H; 16, Ph(CH CO H; 17, CH (CH
9, linoleic acid; 20, 4-CH CO H.
3
; 3, Cu(OTf)
2
; 4, Zn(OTf)
2
; 5, Y(OTf)
SO H; 11, p-TsOH; 12,
; 14, CF CO H; 15,
CO H; 18, oleic acid;
3 3
; 6, La(OTf) ; 7,
acids, including Zn(OTf) , Y(OTf) , and carboxylic acids,
3
3
3
; 8, Yb(OTf)
3
; 9, H SO ; 10, CF
2
4
3
3
increased the fluorescence intensity compared to the reaction
without acid. Acetic acid provided a 2.2-fold greater initial
velocity compared to the same reaction without acid, while
3
3
2
3
2
2
)
2
2
3
2
)
8
2
1
3
C
H
6 4
2
strong acids, such as H
SO
2 4
3 3
, CF SO H, p-TsOH, D-(+)-10-
3
camphorsulfonic acid, and HNO , showed slower velocities.
These pyrrolidine-acid combinations were also screened in
the same way but using a series of different solvents, such
as DMSO, DMF, 1,4-dioxane, acetone, MeCN, THF, PhMe,
i-PrOH, and MeOH. This study revealed DMSO to be the
most effective solvent. We also evaluated different molar
ratios of pyrrolidine to acetic acid and found that 1 or more
equiv of acetic acid was more efficient than combinations
involving less than 1 equiv of acetic acid.
(
3) Selected aldol studies: (a) List, B.; Lerner, R. A.; Barbas, C. F., III.
J. Am. Chem. Soc. 2000, 122, 2395-2396. (b) Sakthivel, K.; Notz, W.;
Bui, T.; Barbas, C. F., III. J. Am. Chem. Soc. 2001, 123, 5260-5267. (c)
Nakadai, M.; Saito, S.; Yamamoto, H. Tetrahedron 2002, 58, 8167-8177.
(
3
d) Cordova, A.; Notz, W.; Barbas, C. F., III. J. Org. Chem. 2002, 67,
01-303. (e) Kotrusz, P.; Kmentova, I.; Gotov, B.; Toma, S.; Solcaniova,
E. Chem. Commun. 2002, 2510-2511. (f) Chowdari, N. S.; Ramachary,
D. B.; Cordova, A.; Barbas, C. F., III. Tetrahedron Lett. 2002, 43, 9591-
9
6
2
595. (g) Anders, B.; Nagaswamy, K.; Anker, J. K. Chem. Commun. 2002,
20-621. (h) Northrup, A. B.; MacMillan, D. W. C. J. Am. Chem. Soc.
002, 124, 6798-6799. (i) Tang, Z.; Jiang, F.; Yu, L.-T.; Cui, X.; Gong,
To determine the utility of these reaction conditions to
intermolecular aldol reactions involving R,R-dialkylaldehyde
donors, the syntheses of a variety of quaternary carbon
L.-Z.; Qiao, A.; Jiang, Y.-Z.; Wu, Y.-D. J. Am. Chem. Soc. 2003, 125,
5
1
262-5263. (j) Darbre, T.; Machuqueiro, M. Chem. Commun. 2003, 1090-
091.
(4) Recent studies in organocatalysis: (a) Pidathala, C.; Hoang, L.;
7,8
Vignola, N.; List, B. Angew. Chem., Int. Ed. 2003, 42, 2785-2788. (b)
containing aldols were evaluated (Table 1). Without acetic
acid, the reaction of isobutyraldehyde (7a) and p-nitro-
benzaldehyde (8a) provided aldol product 9a in 74% yield
after 2 days (entry 1). In contrast, addition of 1 equiv of
acetic acid to pyrrolidine improved the yield to 89% after
only 4 h (entry 2). With 5 equiv of acetic acid with respect
to pyrrolidine, 9a was obtained in 95% yield after only 15
Juhl, K.; Jorgensen, K. A. Angew. Chem., Int. Ed. 2003, 42, 1498-1501.
(
c) Hayashi, Y.; Tsuboi, W.; Ashimine, I.; Urushima, T.; Shoji, M.; Sakai,
K. Angew. Chem., Int. Ed. 2003, 42, 3677-3680. (d) Halland, N.; Aburel,
P. S.; Jorgensen, K. A. Angew. Chem., Int. Ed. 2003, 42, 661-665. (e)
Hoang, L.; Bahmanyar, S.; Houk, K. N.; List, B. J. Am. Chem. Soc. 2003,
1
25, 16-17. (f) Bahmanyar, S.; Houk, K. N.; Martin, H. J.; List, B. J. Am.
Chem. Soc. 2003, 125, 2475-2479. (g) Melchiorre, P.; Jorgensen, K. A. J.
Org. Chem. 2003, 68, 4151-4157. (h) Chowdari, N. S.; Ramachary, D.
B.; Barbas, C. F., III. Org. Lett. 2003, 5, 1685-1688. (i) Bahmanyar, S.;
Houk, K. N. Org. Lett. 2003, 5, 1249-1251. (j) Cordova, A.; Barbas, C.
F., III. Tetrahedron Lett. 2003, 44, 1923-1926. (k) Liu, H.; Peng, L.; Zhang,
T.; Li, Y. New J. Chem. 2003, 27, 1159-1160. (l) Zhong, G. Angew. Chem.,
Int. Ed. 2003, 42, 4247-4250. (m) Ramachary, D. B.; Chowdari, N. S.;
Barbas, C. F., III. Angew. Chem., Int. Ed. 2003, 42, 4233-4237. (n) Hayashi,
Y.; Tsuboi, W.; Shoji, M.; Suzuki, N. J. Am. Chem. Soc. 2003, 125, 11208-
(7) Pyrrolidine-AcOH has been studied in intramolecular aldol reactions;
see ref 6b-d. See also: (a) Desmaele, D.; d’Angelo, J. Tetrahedron Lett.
1989, 30, 345-348. (b) Snider, B. B.; Yang, K. J. Org. Chem. 1990, 55,
4392-4399. (c) Barros, M. T.; Santos, A. G.; Godinho, L. S.; Maycock,
C. D. Tetrahedron Lett. 1994, 35, 3999-4002. (d) Greco, M. N.; Maryanoff,
B. E. Tetrahedron Lett. 1992, 33, 5009-5012.
(8) The fluorescent screening results were roughly correlated to the aldol
reaction results. For example, compared to pyrrolidine-acetic acid (V )
138.8 RFU/s in Figure 1), the pyrrolidine-D-(+)-10-camphorsulfonic acid
combination (V ) 13.5) did not catalyze the aldol reaction after 24 h.
Pyrrolidine-Yb(OTf)3 (V ) 46.2) and pyrrolidine-p-toluic acid (V ) 131.8)
gave the aldol product in 82% (15 h) and 91% (5 h) yield, respectively.
1
1209.
(5) Tanaka, F.; Thayumanavan, R.; Barbas, C. F., III. J. Am. Chem. Soc.
2
003, 125, 8523-8528.
(
Spencer, T. A.; Neel, H. S.; Ward, D. C.; Williamson, K. L. J. Org. Chem.
966, 31, 434-436. (c) Woodward, R. B. Pure Appl. Chem. 1968, 17, 519-
47. (d) Hajos, Z. G.; Parrish, D. R. J. Org. Chem. 1974, 39, 1612-1615.
6) (a) Kuehne, M. E. J. Am. Chem. Soc. 1959, 81, 5400-5404. (b)
1
5
4370
Org. Lett., Vol. 5, No. 23, 2003

Table 1. Pyrrolidine-Acetic Acid Catalyzed Direct Aldol Reactions for the Synthesis of Quaternary Carbon Bearing Aldols
a
Isolated yields of pure product after column chromatography. b 10 equivalent of cyclopropanecarboxaldehyde (7c) was used. Typical procedure: To a
solution of arylaldehyde 8 (0.5 mmol) and R,R-dialkylaldehyde 7 (0.6 mmol) in DMSO (0.5 mL) were added acetic acid and pyrrolidine, in the molar ratios
indicated. The reaction mixture was stirred at room temperature for the indicated time and then purified by flash silica gel column chromatography without
workup to give the aldol product 9.
min (entry 3). When both acetic acid and pyrrolidine were
decreased (entry 4) the yield after 2 h was 96%. All of the
R,R-dialkylaldehydes except 7a required longer reaction
times for their reactions to complete; therefore, we used 0.3
equiv of amine-acid catalyst in the reactions of R,R-
dialkylaldehydes 7b-f with 8a. Except in the case of cyclo-
propanecarboxaldehyde (7c), reactions of cyclohexane-
carboxaldehyde (7b) and/or the R-alkyl-R-methylaldehydes,
such as 2-methylbutyraldehyde (7d), 2,6-dimethyl-5-heptenal
a solution of pyrrolidine (10 µmol) in DMSO-d (500 µL)
6
was added acetic acid (10 µmol). The chemical shift at 2-H
of pyrrolidine was shifted from 2.65 to 2.83 ppm, indicating
the formation of the salt 11. To this solution was added
isobutyraldehyde (10 µmol, -CHO 9.55 ppm), and the
3 2
formation of enamine 13 (-N-CHdC(CH ) 5.56 ppm) was
Scheme 2. Proposed Mechanism of the Pyrrolidine-Acetic
Acid Catalyzed Direct Aldol Reaction
(7e), and 3-(4-isopropylphenyl)isobutyraldehyde (7f), yielded
at least 80% of the expected product within 4 h (entries 5-9).
The reaction of 4-cyanobenzaldehyde (8b) with 7a also
afforded the aldol product 9g in 92% yield (entry 10).
These reaction conditions were readily scaled. To study a
multigram-scale synthesis, acetic acid (0.25 equiv) and
pyrrolidine (0.05 equiv) were added to a solution of
p-nitrobenzaldehyde (10.0 g) and isobutyraldehyde (1.2
equiv) in THF (66 mL) at room temperature. The reaction
mixture was stirred for 2 h and then concentrated to give
the crude product, which was purified by flash silica gel
column chromatography to afford aldol product 9a (14.0 g,
95%).
We confirmed the formation of the presumed enamine
1
intermediate by H NMR for the reaction in Scheme 2. To
Org. Lett., Vol. 5, No. 23, 2003
4371

observed within 5 min. In the absence of acetic acid, enamine
formation was observed after 1 h when the same enamine
chemical shift became predominant. These NMR studies
suggest that acceleration of enamine formation is key to
increasing the rate of this reaction and is therefore at least
partially rate-determining.
can be carried out under simple and mild reaction conditions,
and can be applied to multigram reactions. Further studies
focusing on the full scope of this catalyst system are currently
under investigation and will be reported in due course.
Acknowledgment. This study was supported in part by
the NIH (CA27489) and the Skaggs Institute for Chemical
Biology.
In summary, we have used a rapid fluorescence assay to
determine optimal reaction conditions for intermolecular
direct aldol reactions of R,R-dialkylaldehydes 7 with aryl-
aldehyde 8. The reaction proceeds via an enamine intermedi-
Supporting Information Available: Complete analytical
data for all new compounds. This material is available free
of charge via the Internet at http://pubs.acs.org.
1
ate as shown by H NMR. The pyrrolidine-acetic acid
bifunctional catalyst system utilizes inexpensive chemicals,
does not require preactivation of the donor and/or acceptor,
OL035651P
4372
Org. Lett., Vol. 5, No. 23, 2003