Increase of catalytic activity of alpha-chymotrypsin by metal salts for transesterification of an amino acid ester in ethanol.

Article abstract of DOI:10.1271/bbb.61.1196

alpha-Chymotrypsin-catalyzed transesterification of N-acetyl-L-tyrosine methyl ester in ethanol was markedly accelerated by addition of small amounts of divalent metal salts. The reaction rate dependent not only on the nature of metal ions but also on the nature of anionic counter ions. Calcium acetate was the most effective among the metal salts used. The reaction followed Michaelis-Menten kinetics, and it was found that the reaction increase is due to the increase in kcat.

Full text of DOI:10.1271/bbb.61.1196

This article was downloaded by: [University of Louisville]
On: 03 January 2015, At: 09:47
Publisher: Taylor & Francis
Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer
House, 37-41 Mortimer Street, London W1T 3JH, UK
Bioscience, Biotechnology, and Biochemistry
Publication details, including instructions for authors and subscription information:
http://www.tandfonline.com/loi/tbbb20
Increase of Catalytic Activity of α-Chymotrypsin by
Metal Salts for Transesterification of an Amino Acid
Ester in Ethanol
a
a
Toshiya Sasaki & Hideo Kise
a
Institute of Materials Science, University of Tsukuba, 1–1–1 Tennoudai, Tsukuba, Ibaraki
05, Japan
3
Published online: 12 Jun 2014.
To cite this article: Toshiya Sasaki & Hideo Kise (1997) Increase of Catalytic Activity of α-Chymotrypsin by Metal Salts for
Transesterification of an Amino Acid Ester in Ethanol, Bioscience, Biotechnology, and Biochemistry, 61:7, 1196-1197, DOI:
10.1271/bbb.61.1196
To link to this article: http://dx.doi.org/10.1271/bbb.61.1196
PLEASE SCROLL DOWN FOR ARTICLE
Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained
in the publications on our platform. However, Taylor & Francis, our agents, and our licensors make no
representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of
the Content. Any opinions and views expressed in this publication are the opinions and views of the authors,
and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied
upon and should be independently verified with primary sources of information. Taylor and Francis shall
not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other
liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or
arising out of the use of the Content.
This article may be used for research, teaching, and private study purposes. Any substantial or systematic
reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any
form to anyone is expressly forbidden. Terms & Conditions of access and use can be found at http://
www.tandfonline.com/page/terms-and-conditions

NII-Electronic Library Service

NII-Electronic Library Service