A simple enzymic method for the synthesis of adenosine 5' [α 32P]triphosphate on a preparative scale

Article abstract of DOI:10.1042/bj1610555

A simple, rapid and inexpensive method is described for the enzymic synthesis of (α³²P]ATP from [³²P]Pi on a preparative scale with an overall yield of 53%. The final product contained all of the detectable radioactivity (>99.9%) in the α position and has been shown to behave identically with commercially available [α³²P]ATP during the synthesis of 3':5' cyclic AMP in the reaction catalysed by adenylate cyclase.