Positional specificity and stereoselectivity of a lipase preparation from oat seeds acting on 1,2,3-trihexanoylglycerol

Article abstract of DOI:10.1271/bbb.61.166

Oat seed lipase was extracted with 0.01 M calcium chloride solution containing 0.5% Triton X-100 and precipitated with ammonium sulfate. The precipitate was dissolved in phosphate buffer at pH 6.0 and the supernatant was used as the lipase preparation. The lipase was very selective in the ester positions of 1,2-trihexanoylglycerol, hydrolyzing sn-3 most quickly, sn-1 moderately, and sn-2 hardly at all.

Full text of DOI:10.1271/bbb.61.166

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Positional Specificity and Stereoselectivity of a
Lipase Preparation from Oat Seeds Acting on 1,2,3-
Trihexanoylglycerol
Yasuhide Otaa, Toshio Minesakia & Oshima Akia
a Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima
University, 1–4–4 Kagamiyama, Higashi-Hiroshima, Hiroshima 739, Japan
Published online: 12 Jun 2014.
To cite this article: Yasuhide Ota, Toshio Minesaki & Oshima Aki (1997) Positional Specificity and Stereoselectivity of a
Lipase Preparation from Oat Seeds Acting on 1,2,3-Trihexanoylglycerol, Bioscience, Biotechnology, and Biochemistry, 61:1,
166-167, DOI: 10.1271/bbb.61.166
To link to this article: http://dx.doi.org/10.1271/bbb.61.166
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