Affinity chromatography on immobilised adenosine 5' monophosphate. I. A new synthesis and some properties of an N6 immobilised 5' AMP

Article abstract of DOI:10.1111/j.1432-1033.1974.tb03273.x

N⁶ (6 Aminohexyl) 5' AMP has been prepared by a single step reaction involving the nucleophylic displacement of the thiol group from 6 mercaptopurine riboside 5' phosphate by 1,6 hexane diamine. The structure of the product has been confirmed by physical, chemical and enzymic analyses. The 5' AMP derivative was coupled to Sepharose 4 B and used as an affinity adsorbent in the separation of dehydrogenases and kinases.