A study of the esterification of caffeic acid with methanol using p-toluenesulfonic acid as a catalyst

Article abstract of DOI:10.2298/JSC120802101W

Caffeic acid (CA) could be considered as an important natural antioxidant. However, the low solubility and stability of CA in various solvent systems is a major limiting factor governing its overall application in the lipid industry. However, the synthesi

Full text of DOI:10.2298/JSC120802101W

J. Serb. Chem. Soc. 78 (7) 1023–1034 (2013)
JSCS–4478
UDC 66.095.13:547.587.52+547.261:
547.269.3+547.533+544.973
Original scientific paper
A study of the esterification of caffeic acid with methanol using
p-toluenesulfonic acid as a catalyst
1
*
1
1
1
1,2**
JUN WANG , SHUANGSHUANG GU , NA PANG , FANGQIN WANG and FUAN WU
1School of Biology and Chemical Engineering, Jiangsu University of Science and Technology,
Zhenjiang 212018, P. R. China and 2Sericultural Research Institute, Chinese Academy of
Agricultural Sciences, Zhenjiang 212018, P. R. China
(Received 2 August, revised 24 September 2012)
Abstract: Caffeic acid (CA) could be considered as an important natural anti-
oxidant. However, the low solubility and stability of CA in various solvent
systems is a major limiting factor governing its overall application in the lipid
industry. However, the synthesis of methyl caffeate (MC) using CA and metha-
nol is a feasible way to improve its lipophilicity. Herein, the reaction condi-
tions and kinetic parameters for the synthesis of MC using p-toluenesulfonic
acid (PTSA) as a catalyst were investigated, and the product was confirmed by
liquid chromatography–mass spectrometry (LC–MS), Fourier transform infra-
red (FTIR) and nuclear magnetic resonance (NMR) spectroscopy, and melting
point analysis. The highest yield of MC catalyzed by PTSA attained 84.0 %
under the optimum conditions as follows: molar ratio of methanol to CA of
20:1, reaction temperature of 65 °C, mass ratio of catalyst to substrate of 8 %,
and a reaction time of 4 h. The esterification kinetics of CA and methanol is
described by the pseudo-homogeneous second order reversible model. The
relationship between temperature and the forward rate constant gives the
activation energy of 17.5 kJ mol-1. These results indicated that PTSA possesses
high catalytic activity in the synthesis of MC, which is an efficient catalyst
suitable for MC production in the chemical industry.
Keywords: caffeic acid; catalysis; esterification; kinetics; methyl caffeate;
p-toluenesulfonic acid.
INTRODUCTION
Caffeic acid (CA) is one of the most widely distributed hydroxycinnamate
1,2
and phenylpropanoid metabolites in plant tissues and agricultural wastes.
Recently, CA, as a natural antioxidant, has received increasing attention with
regard to its applications in the food, health, cosmetic and pharmaceutical indus-
tries because of its numerous biological activities, such as anti-mutagenic, anti-
Corresponding authors. E-mails: *jimwang_js@hotmail.com; **fuword@163.com
doi: 10.2298/JSC120802101W
1023
Available online at www.shd.org.rs/JSCS/
___________________________________________________________________________________________________________________
Copyright (C)2013 SCS

1024
WANG et al.
3–5
proliferative and anti-oxidant activities.
However, CA exhibits low solubility
and stability in various solvent systems, thus it is necessary to enhance its prac-
6
tical applicability by improving its solubility. The strategy of esterification of
hydrophilic CA with lipophilic molecules, such as aliphatic alcohols, could be
7–9
employed to alter its solubility in hydrophobic media.
In addition, the syn-
10,11
thesis of alkyl esters can significantly improve their function.
For example, it
was found that alkyl esters of CA have a higher antioxidant activity and lipophi-
12
licity than CA to protect neuronal PC12 cells against oxidative stress. There-
fore, it is advantageous to synthesizе alkyl esters of caffeic acid based on both
their biological function and potential application.
The classical methods for the esterification of CA, such as Fischer esteri-
13–15
fication, Wittig and one-pot reactions,
require tedious operations and gene-
rate by-products. Nowadays, a simple reactive approach appeared, i.e., the direct
esterification of CA with alcohols. Shin et. al. described an esterification of CA
in the presence of methanol and sulfuric acid to prepare methyl caffeate (MC). In
13
the reaction, the reaction time was 10 h and the yield achieved was up to 71 %.
Sulfuric acid has been widely used as an acid catalyst in a large variety of indus-
trial chemical processes. However, sulfuric acid generally shows weak catalytic
16
activity and requires high reaction temperatures and long reaction time. In
addition, the use of sulfuric acid is hazardous and requires special energy-inef-
17
ficient processes for the treatment of the waste acid. Thus, there is an urgent
necessity to find a valid and simple catalyst and esterification system for the
efficient synthesis of MC.
Recently, it was shown that p-toluenesulfonic acid (PTSA) has the potential
18
to be used as a substitute for conventional acidic catalytic materials. It is cha-
racterized by the mildness of the reaction conditions, inexpensive chemical and
the excellent functional group tolerance, allowing the formation of the corres-
19
ponding esters in good to excellent yields. For instance, Jagdale et al. per-
formed hydroarylation of cinnamic acids with anisoles and phenols mediated by
PTSA under metal and solvent-free conditions, affording 3-(4-methoxyphenyl)-
-3-phenylpropanoic acids and dihydrocoumarins, respectively, in high yields with
20
excellent selectivity. Therefore, the use of PTSA as a catalyst is potentially attrac-
21
tive for the synthesis of MC. However, a major concern of this system is its
2
sensitivity and instability, because the esters of CA are readily oxidized. More-
over, nowadays, the pseudo-homogeneous model of esterification is a competent
kinetic model for predicting an esterification system, taking into account that it is
22,23
a less complex model.
Hence, it is necessary to study the reaction conditions
and kinetic parameters for an efficient synthesis of MC catalyzed by PTSA.
In the present study, using PTSA as the catalyst, the factors that strongly
affect the yield of MC (namely, the reaction temperature, catalyst loading, the
mole ratio of methanol to CA, and the ratio of molecular sieve to methanol) were
Available online at www.shd.org.rs/JSCS/
___________________________________________________________________________________________________________________
Copyright (C)2013 SCS

ESTERIFICATION KINETICS OF CAFFEIC ACID
1025
explored and the corresponding esterification kinetic parameters of CA with me-
thanol were obtained by the pseudo-homogeneous model. Liquid chromategra-
phy–mass spectrometry (LC-MS), Fourier transform infrared (FTIR) and nuclear
magnetic resonance (NMR) spectroscopy, and melting point analysis were used
to identify MC in the synthesis product.
EXPERIMENTAL
Materials and reagent
CA (purity > 98 %) was purchased from Nanjing Zelang Pharmaceutical Sci. & Tech.
Co. Ltd. (Nanjing, China). Methanol (HPLC grade) and PTSA were purchased from Sino-
pharm Chemical Reagent Co. Ltd. (Shanghai, China). All other employed solvents and
reagents were of analytical grade. Water was purified using an Elga Purelab Option-Q purify-
cation system (Elga Labwater, High Wycombe, Bucks, UK) and had a resistance greater than
18.0 MΩ cm.
General procedure for MC synthesis
The MC synthesis was performed in 5 mL screw-capped vials containing CA and metha-
nol. The substrate was weighed accurately and the reaction was realized by the addition of
PTSA. The reactions were generally performed under the following condition: 1 mM of CA,
20 mM of methanol, 2 % molecular sieves and 6 % PTSA were mixed and reacted at 60 °C
under stirring (120 rpm). About 20 μL aliquots of the well-stirred reaction mixture were
withdrawn at intervals for analysis.
Kinetics experimental procedure
CA and PTSA were charged into 5 mL screw-capped vials, and then methanol was
added. This was taken as zero time for a run. The initial mole ratio of CA and methanol was
1:20, and the total volume of the reactant was 0.8 mL. About 20 μL of liquid sample was
withdrawn from the vials at regular intervals for high performance liquid chromatography
(HPLC) analysis. In a typical run, about 10 samples were taken from the system. The sample
injection volume for the HPLC analysis was 20 μL. All samples were determined in triplicate.
The CA conversion and MC yield were calculated as follows:
Consumptive amount of CA, mol
CA Conversion, %=
×100
×100
(1)
(2)
Initial amount of CA, mol
Amount of methyl caffeate, mol
Initial amount of caffeic acid, mol
MC yield, % =
LC–MS and HPLC analysis
LC-PAD–MS was realized on a Thermo Fisher LC-MS system. The LC equipment com-
prised a Finnigan MAT Spectra System P4000 pump, an autosampler with 50 μL loop, a
UV6000LP diode array detector, and a Finnigan AQA mass spectrometer. The LC separation
was accomplished on a Kromasil C18 column (150 mm×4.6 mm, i.d.; 5 μm, W. R. Grace &
Co., Deerfield, Illinois, USA) at 40 °C. The mobile phase consisted of solvent A (methanol)
and solvent B (water). The samples were eluted isocratically using a 65:35 volume ratio of
methanol and water at a flow rate of 1 mL min-1. The wavelength range of the detection was
from 200 to 400 nm. The electrospray ionization (ESI) was performed using nitrogen at a flow
rate of 1.0 mL min-1 to assist nebulization. Selected ion monitoring (SIM) was in the negative
ion mode. A capillary voltage at 1.6 kV was used and the temperature of the curved desol-
Available online at www.shd.org.rs/JSCS/
___________________________________________________________________________________________________________________
Copyright (C)2013 SCS

1026
WANG et al.
vation line (CDL) and heat block were both set at 200 °C. The data were processed using
Xcalibur 1.2 software.
LC–UV was performed using an LB-5 pump (Beijing Satellite Manufactory, Beijing,
China) with a UV detector (L-7420, Techcomp Co. Ltd., Shanghai, China) and N-2000 work-
station (Hangzhou Mingtong S&T, Hangzhou, China). The reaction samples were analyzed on
a C18 column (250 mm×4.6 mm, i.d.; 5 μm, W. R. Grace & Co., Deerfield, Illinois, USA)
maintained at 30 °C. The mobile phase consisted of methanol and water (65:35 volume ratio)
at a flow rate of 1.0 mL min-1, and the detection wavelength was 325 nm.
FTIR, NMR and melting point analysis of MC
MC was prepared from CA as follows: a solution of CA (9 g) in methanol (100 mL) was
treated with a catalytic amount of PTSA and heated at reflux for 6 h. The reaction mixture was
cooled to room temperature and concentrated. The residue was dissolved in ethyl acetate and
washed successively with dilute saturated aqueous NaHCO3 solution, saturated aqueous NaCl,
dried over MgSO4, filtered and evaporated. The residue was crystallized from methanol to
give a pure sample (yield 6.3 g, 70 %) as a white solid, and then was identified as MC by
FTIR and NMR spectroscopy and melting point determination. Before testing, the sample was
dried at 100 °C for 5 h in an oven, and then sealed in plastic bags. A sample was ground into a
fine powder using an agate mortar immediately prior to analysis. The FTIR analysis was
performed at room temperature on a Nicolet 560 instrument using the KBr disc technique. The
1H-NMR spectra were recorded on Bruker Advance Spectrospin 400 MHz using TMS as the
internal standard. The melting point analysis was performed using an Optimelt MPA100
instrument.
RESULTS AND DISCUSSION
LC–MS analysis of the reaction product of CA and methanol
Based on preliminary results, CA had been completely converted to MC
after the esterification reaction of CA and methanol catalyzed by PTSA had pro-
ceeded for 5 h. The high catalytic efficiency of PTSA was proved via LC–MS in
the present work. The LC–UV and TIC chromatograms of the reaction mixture
from reaction of CA and methanol catalyzed by the PTSA are shown in Figs. 1A
and 1B, respectively, which indicated that 84.0 % of the CA was effectively
transformed to MC in the presence of methanol. The ESI-MS spectra of the reac-
tion mixture are presented in Figs. 1C and 1D, which exhibit two intense peaks at
–
m/z 179.01 and 193.07, corresponding to the deprotonated ion [M–H] of CA and
24
MC, respectively. These results indicated that no side-products were formed
during the whole reaction process catalyzed by the PTSA.
FTIR, NMR and melting point analysis
That MC was obtained in the studied reaction was confirmed by FTIR and
NMR spectroscopy, and melting point analysis.
–1
M.p.: 159–160 °C. FTIR (KBr, cm ): 3477, 3099, 2925, 1677, 1606, 1536;
1
H-NMR (400 MHz, DMSO-d , δ / ppm): 9.60 (1H, s, OH), 9.16 (1H, s, OH),
6
7.50 (1H, d, J = 15.9 Hz, α-H), 7.07 (1H, d, J = 2.0 Hz, Ph–H), 7.01 (1H, dd, J =
Available online at www.shd.org.rs/JSCS/
___________________________________________________________________________________________________________________
Copyright (C)2013 SCS

ESTERIFICATION KINETICS OF CAFFEIC ACID
1027
= 8.1 Hz, Ph–H), 6.77 (1H, d, J = 8.1 Hz, Ph–H), 6.28 (1H, d, J = 15.9 Hz, β-H),
3.69 (3H, s, CH ).
3
Fig. 1. Analysis of the reaction mixture after CA esterification with methanol catalyzed by
PTSA: A) LC–UV chromatogram, B) TIC chromatogram, C) mass spectrogram, m/z ratio
179.01, and D) mass spectrogram m/z ratio 193.07.
Available online at www.shd.org.rs/JSCS/
___________________________________________________________________________________________________________________
Copyright (C)2013 SCS

1028
WANG et al.
Effect of mole ratio of methanol to CA on the yield of MC
The esterification reaction between CA and methanol follows a reversible
path. A higher equilibrium conversion can only be obtained if the backward
reaction is minimized. There are two ways to reduce the rate of backward reac-
tion: a) to remove one of the undesired products, i.e., water in the present case,
25
continuously or b) to use one of the reactants in excess (methanol in this case).
In the present system, it was not easy to remove water, as the employed system
was a closed system. Thus, the option of using excess methanol was employed in
the present study. The change of the MC yield with the mole ratio of methanol to
CA is shown in Fig. 2A. The MC yield increased from 25.8 % to 71.7 % on
increasing the mole ratio of methanol to CA from 5:1 to 20:1. According to the
Le Chatelier principle, the excess methanol was used to shift the equilibrium of
26
the reversible reaction toward the direction of ester formation, while any fur-
ther increase exhibited an inhibitory effect on the production of MC. At a mole
ratio of 50:1, the yield of MC was significantly lower because the use of too high
amount of methanol could increase the water content, which arises from com-
27
mercial methanol. Therefore, the optimal mole ratio of methanol to CA for MC
production was suggested to be 20:1.
Effect of reaction temperature on the yield of MC
The reaction temperature is one of the most important parameter affecting
the MC yield during the esterification process. Six different temperatures were
selected in the range of 45–70 °C. The MC yield markedly increased from 48.9
% to 78.9 % on increasing the temperature from 45 to 65 °C (see Fig. 2B).
Obviously, a higher temperature results in an increase in the reaction rate and the
17
equilibrium constant for an endothermic reaction. With a further increase to 70
°C, the final yield of MC decreased to 65.4 %. These results indicated that higher
temperatures could activate the substrate molecules, reduce the viscosity of the
reaction mixture and lead to a higher reaction rate. However, too high a tempera-
28
ture leads to methanol volatilization. Therefore, a temperature of 65 °C was
retained in the subsequent optimization experiments.
Effect of catalyst loading on the yield of MC
To study the effect of catalyst loading on the yield of methyl ester, the reac-
tion was performed at seven different mass ratios of catalyst to substrate (1, 2, 4,
6, 8 and 10 %), the other reaction conditions being kept constant. The results are
shown in Fig. 2C, which indicates that there was a significant increase in the
yield of MC as the mass ratio of catalyst to substrate increased from 0 % to 8 %,
under identical conditions. The amount of PTSA influences the reaction rate because
+
more H become available when the amount of catalyst in the mixture in-
29
creases. Unexpectedly, when the mass ratio of catalyst to substrate was in-
Available online at www.shd.org.rs/JSCS/
___________________________________________________________________________________________________________________
Copyright (C)2013 SCS

ESTERIFICATION KINETICS OF CAFFEIC ACID
1029
Fig. 2. The effects on the yield of MC of A) the mole ratio of methanol to CA (5:1 to 100:1) at
a mass ratio of catalyst to substrate of 6 % and a temperature of 60 °C, B) reaction
temperature, C) catalyst loading and D) mass ratio of molecular sieve to methanol, on the
yield of MC catalyzed by PTSA. Reaction conditions: B) mole ratio of methanol to CA of
20:1 and a mass ratio of catalyst to substrate of 5 %, C) the catalyst loading (0 to 10 %) at a
mole ratio of methanol to CA 20:1 and a temperature of 65 °C and D) the mass ratio of
molecular sieve to methanol (0 to 15 %) at a molar ratio of methanol to CA of 20:1, a mass
ratio of catalyst to substrate of 8 % and temperature of 65 °C.
creased to 10 %, the yield of MC decreased. Similar results were reported for the
synthesis of functionalized pyrimido[4,5-b]quinolines and indeno-fused pyrido-
30
[2,3-d]pyrimidines in water catalyzed by PTSA. Therefore, the optimum mass
ratio of catalyst to substrate for MC synthesis was 8 %.
Effect of mass ratio of molecular sieve to substrate on the yield of MC
Water is the second product during the esterification of CA with methanol.
Thus, the water content is another important parameter in esterification reactions.
Kumar et. al. reported that addition of molecular sieves or silica usually improves
16
the equilibrium conversion. To study the effect of the reaction water on the
reaction rate, 0–15 % 3 Å molecular sieves were added to remove the water. Fig.
2D showed that the yield of MC decreased with increasing ratio of molecular
Available online at www.shd.org.rs/JSCS/
___________________________________________________________________________________________________________________
Copyright (C)2013 SCS

1030
WANG et al.
sieve to methanol. This is because PTSA has strong acidic properties and could
corrode the molecular sieves in some way. Kuwabara et. al. also reported that
molecular sieves in many cases had negative effects, such as the formation of di-
31
esters and degradation of unstable substances. Therefore, the employment of
molecular sieves to remove the formed water is not suitable here.
Kinetics of the esterification of CA with methanol in the presence of PTSA
The effect of the reaction time on the esterification of CA with methanol
catalyzed by PTSA at different temperatures is shown in Fig. 3A. With increasing
time, the conversion of CA increased rapidly, which reflects the synthesis ability
and stability of MC in the PTSA-catalyzed reaction system. After an esterifica-
tion time of 4 h, the conversion of CA reached a stable value. Therefore, the
optimal reaction time was 4 h. The highest yield of MC was 84.0 % under the
optimum conditions as follows: mole ratio of methanol to CA of 20:1, reaction
temperature of 65 °C, reaction time of 4 h, and mass ratio of catalyst to substrate
of 8 %.
Fig. 3. Second order reversible
model for the calculation of the
reaction rate constants at different
reaction temperatures.
Available online at www.shd.org.rs/JSCS/
___________________________________________________________________________________________________________________
Copyright (C)2013 SCS

ESTERIFICATION KINETICS OF CAFFEIC ACID
1031
The pseudo-homogeneous model does not take into account the sorption
32,33
effect into the catalyst in a reactant medium.
If the reaction mixture is con-
sidered as a single liquid phase, the performance of the pseudo-homogeneous
model could be considered as a satisfactory tool to correlate the esterification of
CA with methanol in the presence of PTSA as a catalyst.
The esterification of CA with methanol using PTSA as a catalyst is repre-
sented as follows:
k1
⎯⎯→
A+B
E+ W
(3)
←⎯⎯
k-1
where A is CA, B is methanol, E is MC and W is water. This reaction can be
considered a second order reaction. Therefore, the rate equation can be written as
follows:
dc
A
r =
A
= k c c − k c c
−1 Ε W
(4)
1 Α Β
dt
where c , c , c and c are the concentrations of CA, methanol, MC and water,
A
B
E
W
respectively. k is the forward rate constant and k is the backward rate constant.
1
–1
From the relationship of c and the extent of conversion of CA, x :
A
A
dc
dx
Α
dt
Α
−
= c
Α0
dt
as well as the correlations of the changes in B, E and W to A:
c = c (1− x )
Α
Α0
Α
c = c (M − x )
Β
Β0
Α
and
c = c = c x
Α0 Α
Ε
w
where c is the initial concentration of CA, c is the initial concentration of
A0
B0
methanol and M is the concentration ratio of methanol to CA (M = c /c ).
B0 A0
Thus, Eq. (4) becomes:
dx
Α
2
= c [k (1− x )(M − x ) − k x ]
(5)
Α0
1
Α
A
−1
A
dt
At equilibrium, dc /dt = 0 and thus, the equilibrium constant (K ) can be
A
e
calculated from:
2
x
k
1
Ae
K =
e
=
(6)
k
(1− x )(M − x
)
−1
Αe
Αe
x
Ae
is the conversion of CA at equilibrium stage, and K is the equilibrium cons-
e
tant.
Available online at www.shd.org.rs/JSCS/
___________________________________________________________________________________________________________________
Copyright (C)2013 SCS

1032
WANG et al.
If Eq. (6) is integrated and rearranged, the following equation in its linear
form is obtained:




 2a x − M −1− a  −M −1+a 
1 Α
2
2
ln
= a k c
t
(7)
2 1 Α0





2a x − M −1+a
−M −1− a

1 Α
2 
2 
where:



1
a = 1−


1
K
e 
1/2
2


a = M +1 − 4a M
(
)
2
1




The results of plotting the left-hand-side of Eq. (7) vs. t are presented in Fig.
3B, from which it can be seen that straight lines passing through the origin were
2
obtained. All straight lines gave a good linear correlation lying in the R range of
0.979–0.989. This clearly shows that the proposed kinetic model is appropriate
for this reaction. The forward reaction rate constant, k , was obtained from the
1
slope of the lines in Figure 3B at different temperatures. The backward rate cons-
tants k could be calculated using the correlation of the forward rate constant
–1
and the equilibrium constant, as in Eq. (7). All forward and backward rate cons-
tants at the different reaction temperatures are given in Table I.
TABLE I. Estimated forward and backward rate constants (L mol-1 min-1) at different reaction
temperatures
Run
Reaction temperature, °C Forward rate constant, k1 Backward rate constant, k-1
1
2
3
50
60
65
0.219
0.376
0.417
2.992
1.749
4.344
The dependence of the forward rate constant on the reaction temperature is
described by the Arrhenius Law, as given in Eq. (8):
−E/RT
k = Ae
1
(8)
where, A is the pre-exponential factor, E is the activation energy, R is the idea gas
constant and T is the reaction temperature (K).
The Arrhenius–Van’t Hoff plot of the forward rate constant in the tempera-
ture range 50–65 °C gives the coefficient of linearity of 0.940. The slope could
–1
be applied to calculate the activation energy. The activation energy is 17.5 kJ mol ,
which confirms that PTSA possesses a high catalytic activity in the synthesis of MC.
CONCLUSIONS
The reaction conditions and kinetic parameters for the efficient synthesis of
MC by esterification of CA by methanol using PTSA as a catalyst were first
Available online at www.shd.org.rs/JSCS/
___________________________________________________________________________________________________________________
Copyright (C)2013 SCS

ESTERIFICATION KINETICS OF CAFFEIC ACID
1033
investigated. The results indicated that the highest attained yield of MC was 84.0
% under the optimum conditions, i.e., reaction time of 4 h, reaction temperature
of 65 °C, mole ratio of methanol to CA of 20:1, and mass ratio of catalyst to
substrate of 8 %. A pseudo-homogeneous second order reversible model was
demonstrated to justify the experimental data. The model provided good agree-
ment with the experimental kinetic results. The activation energy of the reaction
–1
was 17.5 kJ mol , which indicates that PTSA possesses high catalytic activity in
the synthesis of MC.
Acknowledgements. This work was sponsored by Natural Science Foundation of Jiangsu
Province (BK2009213), National Natural Science Foundation of China (21206061), China
Postdoctoral Science Foundation funded projects (2012M510124, 2013T60505), Qing Lan
Project of Jiangsu Province, Postdoctoral Science Foundation Funded Project of Jiangsu
University (1143002085), Graduate Innovation Project of Jiangsu Province (2012JSSPITP1482),
Graduate Innovation Project of Jiangsu University of Science and Technology, Research
Projects of Jiangsu University of Science and Technology (35211002, 33201002), and
Modern Agro-industry Technology Research System of China (CARS-22).
The authors have declared no conflict of interest.
И З В О Д
ИСПИТИВАЊЕ РЕАКЦИЈЕ ЕСТЕРИФИКАЦИЈЕ КOФЕИНСКЕ КИСЕЛИНЕ СА
МЕТАНОЛОМ У ПРИСУСТВУ p-ТОЛУЕНСУЛФОНСКЕ КИСЕЛИНЕ КАО
КАТАЛИЗАТОРА
JUN WANG1, SHUANGSHUANG GU1, NA PANG1, FANGQIN WANG1 и FUAN WU1,2
1
School of Biology and Chemical Engineering, Jiangsu University of Science and Technology,
2
Zhenjiang 212018, P.R. China и Sericultural Research Institute, Chinese Academy
of Agricultural Sciences, Zhenjiang 212018, P.R. China
Кoфеинска киселина (KK) представља важан природни антиоксидант. Слаба рас-
творљивост и стабилност KK у различитим растварачима представља главни огранича-
вајући фактор свеукупне примене у индустрији липида. Због тога синтеза метил-кoфе-
ата (MK) из KK и метанола представља могући начин да се побољша липофилност. У
оквиру рада, прво су испитани реакциони услови и кинетички параметри синтезе MK
користећи p-толуенсулфонску киселину (PTSA) као катализатор. У овом раду су прво
испитивани реакциони услови и кинетички параметри при синтези MK и за идентифи-
кацију производа су коришћене методе LC–MS, FTIR, NMR и анализа тачке топљења.
Највећи остварени принос MK у присуству PTSA је био 84 %, а оптимални реакциони
услови су били: молски однос метанол: KK 20:1, реакциона температура 65◦°C, масени
однос катализатор : супстрат 8 %, и реакционо време 4 h. Реакција естерификације KK
метанолом је описана ревирзибилним моделом псеудо-хомогеног другог реда. На
основу зависности константе k1 од температуре активациона енергија износи 17,5 kJ
mol-1. Резултати показују да PTSA поседује каталитичку активност у синтези МК, као и
да је ефикасан катализатор погодан за синтезу MK у хемијској индустрији.
(Примљено 2. августа, ревидирано 24. септембра 2012)
REFERENCES
1. J. Fu, K. Cheng, Z. Zhang, R. Fang, H. Zhu, Eur. J. Med. Chem. 45 (2010) 2638
Available online at www.shd.org.rs/JSCS/
___________________________________________________________________________________________________________________
Copyright (C)2013 SCS

1034
WANG et al.
2. J. Wang, D. Lu, H. Zhao, X. Ling, B. Jiang, P. Ouyang, Afr. J. Biotech. 8 (2009) 1416
3. Y. Tsai, S. Chiou, K. Chan, J. Sung, S. Lin, LWT – Food Sci. Technol. 46 (2012) 169
4. T. Nagaoka, A. H. Banskota, Y. Tezuka, I. Saiki, S. Kadota, Bioorg. Med. Chem. 10
(2002) 3351
5. A. O. Aytekin, S. Morimura, K. Kida, J. Biosci. Bioeng. 111 (2011) 212
6. H. Chen, Y. Twu, C. J. Chang, Y. Liu, C. Shieh, Ind. Crop Prod. 32 (2010) 522
7. K. Uwai, Y. Osanai, T. Imaizumi, S. Kanno, M. Takeshita, M. Ishikawa, Bioorg. Med.
Chem. 16 (2008) 7795
8. J. Wang, S. Gu, L. Zhang, F. Wu, X. Guo, Acta Crystallogr., E 67 (2011) O2871
9. S. Gu, J. Wang, F. Pan, N. Pang, F. Wu, Acta Crystallogr., E 68 (2012) O557
10. X. S. Zhou, J. B. Liu, W. F. Luo, Y. W. Zhang, H. Song, J. Serb. Chem. Soc. 76 (2011)
1607
11. Z. Knezevic-Jugovic, D. Bezbpadica, Z. Jakovijevic, S. Brankovic-Dimitrijevic, D. Mijin,
J. Serb. Chem. Soc. 73 (2008) 1139
12. J. Garrido, A. Gaspar, E. M. Garrido, R. Miri, M. Tavakkoli, S. Pourali, L. Saso, F.
Borges, O. Firuzi, Biochimie 94 (2012) 961
13. K. Shin, I. Kim, Y. Park, J. Ha, J. Choi, H. Park, Y. S. Lee, K. Lee, Biochem. Pharmacol.
68 (2004) 2327
14. F. de Campos Buzzi, C. L. Franzoi, G. Antonini, M. Fracasso, V. C. Filho, R. A. Yunes,
R. Niero, Eur. J. Med. Chem. 44 (2009) 4596
15. S. M. Fiuza, C. Gomes, L. J. Teixeira, M. T. Girão Da Cruz, M. N. D. S. Cordeiro, N.
Milhazes, F. Borges, M. P. M. Marques, Bioorg. Med. Chem. 12 (2004) 3581
16. A. Kumar, S. S. Kanwar, Bioresour. Technol. 102 (2011) 2162
17. Y. Feng, B. He, Y. Cao, J. Li, M. Liu, F. Yan, X. Liang, Bioresour. Technol. 101 (2010)
1518
18. L. Xiao, C. Xia, J. Chen, Tetrahedron Lett. 48 (2007) 7218
19. G. Le Bras, O. Provot, J. Peyrat, M. Alami, J. Brion, Tetrahedron Lett. 47 (2006) 5497
20. A. R. Jagdale, A. Sudalai, Tetrahedron Lett. 48 (2007) 4895
21. Y. H. Kuo, M. J. Su, US 20090143397 (2009)
22. E. Ö. Akbay, M. R. Altıokka, Appl. Catal. A 396 (2011) 14
23. B. Bessling, J. Löning, A. Ohligschläger, G. Schembecker, K. Sundmacher, Chem. Eng.
Technol. 21 (1998) 393
24. Y. Lu, L. Y. Foo, H. Wong, Phytochemistry 52 (1999) 1149
25. V. G. Deshmane, P. R. Gogate, A. B. Pandit, Ultrason. Sonochem. 16 (2009) 345
26. C. Su, C. Fu, J. Gomes, I. Chu, W. Wu, AIChE J. 54 (2008) 327
27. L. H. Chin, A. Z. Abdullah, B. H. Hameed, Chem. Eng. J. 183 (2012) 104
28. Y. Feng, A. Zhang, J. Li, B. He, Bioresour. Technol. 102 (2011) 3607
29. M. C. de Jong, R. Feijt, E. Zondervan, T. A. Nijhuis, A. B. de Haan, Appl. Catal., A 365
(2009) 141
30. G. K. Verma, K. Raghuvanshi, R. Kumar, M. S. Singh, Tetrahedron Lett. 53 (2012) 399
31. K. Kuwabara, Y. Watanabe, S. Adachi, K. Nakanishi, R. Matsuno, Biochem. Eng. J. 16
(2003) 17
32. J. Lilja, D. Y. Murzin, T. Salmi, J. Aumo, P. Mäki-Arvela, M. Sundell, J. Mol. Catal., A
182–183 (2002) 555
33. B. K. Adnadjević, J. D. Jovanović, Chem. Eng. Technol. 35 (2012) 761.
Available online at www.shd.org.rs/JSCS/
___________________________________________________________________________________________________________________
Copyright (C)2013 SCS

Copyright of Journal of the Serbian Chemical Society is the property of National Library of
Serbia and its content may not be copied or emailed to multiple sites or posted to a listserv
without the copyright holder's express written permission. However, users may print,
download, or email articles for individual use.