BIOSYNTHESIS OF AXENOMYCIN D: INCORPORATION OF 13C-LABELLED PRECURSORS INTO THE MENADIONE CHROMOPHORE, DESOXYSUGARS AND THE AXENOLIDE

Article abstract of DOI:10.1016/S0031-9422(00)95145-8

Experiments on the biosynthesis of the menadione chromophore of axenomycin D in Streptomyces lisandri resulted in the specific incorporation of label from L-<13C>methionine, <1-13C>propionate, <2,3-13C2>propionate, <3-13C>propionate, glycerol and glucose.The data indicate that the menadione chromophore is derived from a metabolite of the shikimate pathway.The mode of incorporation shows, however, that the biosynthesis of the menadione chromophore of axenomycin differs from the biosynthesis of the menadione chromophore of menaquinones.Key Word Index: Streptomyces lisandri; axenomycin; biosynthesis; 13C-NMR spectroscopy.