IDENTIFICATION OF NAA-L-ASPARTATE AMIDE AS THE MAJOR METABOLITE SYNTHESIZED BY TOBACCO MESOPHYLL PROTOPLASTS INCUBATED IN THE PRESENCE OF THE AUXIN ANALOGUE NAA

Article abstract of DOI:10.1016/S0031-9422(00)80429-X

A major metabolite of naphthalene 1-acetic acid (NAA) is rapidly accumulated by tobacco mesophyll protoplasts induced to divide by this growth regulator.A comparison of the natural product with various chemically synthesized NAA-amino acid conjugates was performed.Themetabolite was identified as NAA-aspartate amide by negative CIMS.The biological activity of NAA-aspartate on protoplasts was further studied.The significance of the accumulation of this metabolite in dividing protoplasts is descussed.Key words: Nicotiana tabacum; Solanaceae; protoplast; NAA; auxin-amino acid; conjugates; isolation; identification.