Production of pyridoxal phosphate by a mutant strain of Schizosaccharomyces pombe.

Article abstract of DOI:10.1271/bbb.65.1789

Conditions for extracellular production of vitamin B6 compounds (B6), especially pyridoxal 5'-phosphate (PLP) by Schizosaccharomyces pombe leul strain were examined. The productivity was dependent on concentration of L-leucine in the culture medium: 30 mg/l gave the highest concentrations of total B6 and PLP. The viable cells harvested at different growth phases showed different productivity: middle and late exponential phase cells showed the highest productivity of total B6 and PLP, respectively. D-Glucose (1%, w/v) among other sugars gave the best productivity. Supplementation of air and ammonium sulfate significantly increased extracellular production of PLP. Superoxide anion producers, menadione and plumbagin, and H202 increased the productivity of PLP. Cycloheximide inhibited the increase of PLP by the oxidative stress and, in contrast, increased pyridoxine.

Full text of DOI:10.1271/bbb.65.1789

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Production of Pyridoxal Phosphate by a Mutant Strain
of Schizosaccharomyces pombe
Ruamsub CHUMNANTANAa, Kumi HIROSEa, Hiromichi BABAa & Toshiharu YAGIa
a Department of Bioresources Science, Faculty of Agriculture, Kochi University
Published online: 22 May 2014.
To cite this article: Ruamsub CHUMNANTANA, Kumi HIROSE, Hiromichi BABA & Toshiharu YAGI (2001) Production of
Pyridoxal Phosphate by a Mutant Strain of Schizosaccharomyces pombe, Bioscience, Biotechnology, and Biochemistry,
65:8, 1789-1795, DOI: 10.1271/bbb.65.1789
To link to this article: http://dx.doi.org/10.1271/bbb.65.1789
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