Rate-limiting one-electron transfer in the oxidation of NADH by polyoxometalates

Article abstract of DOI:10.1016/0009-2614(95)00331-W

The kinetics of NADH oxidation by 3 Dawson-type mixed heteropolyanions were studied in buffered aqueous pH = 7 medium, by the stopped flow technique and UV-visible spectroscopy.The log of k was a linear function of the E⁰ of the first redox systems of the heteropolyanions with a slope of 16.5 V^-1.The results indicate that, in the present case, the oxidation of NADH proceeds by a multistep mechanism involving initial rate-limiting one-electron transfer.An estimate of the E⁰ value for the one-electron NADH/NADH^{cation radical} couple has been obtained.