Efficient bioluminescence of bisdeoxycoelenterazine with the luciferase of a deep-sea shrimp Oplophorus

Article abstract of DOI:10.1016/S0040-4039(97)01438-X

The luminescence of the bisdeoxy analogue of coelenterazine (1b : X=Y=H) catalyzed by Oplophorus luciferase was highly efficient (0.19 quanta/molecule), almost comparable to the luminescence of unmodified coelenterazine(1a : X=Y=OH), whereas the luminescence of 1b was very inefficient when catalyzed by apoaequorin or Renilla luciferase and when the arquorin regenerated with 1b was luminesced with Ca²⁺.