Identification of 3,6,7,12-tetrahydroxy-5β-cholan-24-oic acids in human biologic fluids

Article abstract of DOI:10.1016/0039-128X(90)90090-X

Unusual bile acids, 3α,6α,7α,12α-, 3α,6β,7β,12α-, and 3α,6β,7β,12α-tetrahydroxy-5β-cholan-24-oic acids, were identified in all amniotic fluid (four samples) and urine (six samples) from adult patients with cholestalic liver disease by gas-liquid chromatography/mass spectrometry. For the certain identification of these bile acids in the biologic samples, the chemical syntheses of 3α,6β,7α,12α- and 3 α,6β,7β,12α-tetrahydroxy-5β-cholan-24-oic acids were conducted. (Steroids 55:512-515, 1990).