Analytical Chemistry p. 2177 - 2184 (1980)
Update date:2022-08-16
Topics:
Ryan, Mary Andrieu
Ingle, J. D.
The reaction involving the oxidation of thiamine by Hg2+ in basic solutions to fluorescent thiochrome was studied.The initial rate of the reaction, measured as a change in fluorescence signal over 16 s, is linearily related to the thiamine concentration from a detection limit of 2 * 10-8 M up to 1 * 10-4 M.The applicability of the new method for determination of thiamine in vitamin-mineral preparations is demonstrated by investigation of the effect of potential interferences and by analysis of commercial and synthetic preparations.
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