Fluorometric Reaction Rate Method for the Determination of Thiamine

Article abstract of DOI:10.1021/ac50063a042

The reaction involving the oxidation of thiamine by Hg²⁺ in basic solutions to fluorescent thiochrome was studied.The initial rate of the reaction, measured as a change in fluorescence signal over 16 s, is linearily related to the thiamine concentration from a detection limit of 2 * 10^-8 M up to 1 * 10^-4 M.The applicability of the new method for determination of thiamine in vitamin-mineral preparations is demonstrated by investigation of the effect of potential interferences and by analysis of commercial and synthetic preparations.