Perform the phosphorylation reaction using a phosphoramidite derivative of the nucleoside. Phosphoramidites are used in solid-phase oligonucleotide synthesis and are designed to selectively react with the 5' hydroxyl group of the nucleoside, avoiding the N atom. Typical phosphoramidite reagents are activated with activators like tetrazole or activator solution, which can promote selective O-phosphorylation.
If you need to purchase chemical raw materials, submit your demand information to get the latest quotations.
C55H46O12
2-phenyl-2-(S-α-hydroxybenzyl)-1,3-dioxolan
dimethyl 2-[(3-amino-4-oxo-3,4-dihydro-2-quinazolinyl)sulfanyl]-3-(triphenylphosphoranylidine)-butanedioate
N1-(2-thioxo-2,3-dihydro-2λ5-pyrido[2,3-d][1,3,2]oxazaphosphol-2-yl)-4-(4-chlorophenyl)-1-piperazinecarboximidamide
4-METHOXY-1H-PYRAZOLO[3,4-D]PYRIMIDIN-6-AMINE
8-hydroxy-5-(4-cyclohex-1-enyl-1H-1,2,3-triazol-1-yl)quinoline
tetrahydrofurfuryl benzyl phosphorochloridate
2-(3-methyl-2,5-dioxopyrrolidin-1-yl)benzoic acid
N-(5-bromopyridin-3-yl)-4-methoxybenzenesulfonamide
Octadecanoic acid (R)-2-benzyloxy-3-oxo-propyl ester
TRIMETHYLAMINE
Potassium hydroxide
