7660-25-5Relevant articles and documents
NOVEL METHODS OF ISOMERIZING CARBOHYDRATES
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Paragraph 0064; 0065; 0066; 0067; 0069; 0070; 0072-0079, (2017/07/31)
The invention provides a method of isomerizing a sugar into fructose using a calcium salt and an organic base. In certain embodiments, the sugar is glucose and/or mannose.
Enzymatic production and complete nuclear magnetic resonance assignment of the sugar lactulose
Mayer, Juergen,Conrad, Juergen,Klaiber, Iris,Lutz-Wahl, Sabine,Beifuss, Uwe,Fischer, Lutz
, p. 6983 - 6990 (2007/10/03)
The enzymatic transgalactosylation from lactose to fructose leading to the prebiotic disaccharide lactulose was investigated using the β-galactosidase from Aspergillus oryzae and the hyperthermostable β-glycosidase from Pyrococcus furiosus (CelB). The conditions for highest lactulose yields relative to the initial lactose concentration were established on a 1 mL scale. Dependent on the initial molar ratio of lactose to fructose, more or fewer oligosaccharides other than lactulose were generated. Bioconversions on a 30 mL scale in a stirred glass reactor were performed, and lactulose yields of 46 mmol/L (44% relative to lactose) for CelB and 30 mmol/L (30% relative to lactose) for A. oryzae β-galactosidase were achieved. Only 5% of other oligosaccharides were detectable. The corresponding productivities were 24 and 16 mmol/L/h, respectively. The molecular structure of lactulose was investigated in detail and confirmed after purification of the reaction solution by LC-MS and 1D and 2D NMR. Lactulose (4-O-β-D-galactopyranosyl-D-fructose) was unambiguously proved to be the major transglycosylation disaccharide.
Kontinuierliche enzymatische Synthese von 2'-Desoxythymidin-5'-(α-D-glucopyranosyl)diphosphat
Zervosen, Astrid,Elling, Lothar,Kula, Maria-Regina
, p. 592 - 593 (2007/10/02)
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