Characterization of aklavinone (cas 16234-96-1)-11-hydroxylase from Streptomyces purpurascens

Add time:07/29/2019    Source:sciencedirect.com

Aklavinone-11-hydroxylase (RdmE) is a FAD monooxygenase participating in the biosynthesis of daunorubicin, doxorubicin and rhodomycins. The rdmE gene encodes an enzyme of 535 amino acids. The sequence of the Streptomyces purpurascens enzyme is similar to other Streptomyces aromatic polyketide hydroxylases. We overexpressed the gene in Streptomyces lividans and purified aklavinone-11-hydroxylase to apparent homogeneity with four chromatographic steps utilizing a kinetic photometric enzyme assay. The enzyme is active as the monomer with a molecular mass of 60 kDa; it hydroxylates aklavinone and other anthracyclinones. Aklavinone-11-hydroxylase can use both NADH and NADPH as coenzyme but it is slowly inactivated in the presence of NADH. The apparent Km for NADPH is 2 mM and for aklavinone 10 μM. The enzyme is inactivated in the presence of phenylglyoxal and 2,3-butanedione. NADPH protects against inactivation of aklavinone-11-hydroxylase by phenylglyoxal.

We also recommend Trading Suppliers and Manufacturers of aklavinone (cas 16234-96-1). Pls Click Website Link as below: cas 16234-96-1 suppliers

Prev:Antiproliferative activities of halogenated thieno[3,2-d]pyrimidines
Next:Structural Basis for Substrate Recognition and Specificity in aklavinone (cas 16234-96-1)-11-Hydroxylase from Rhodomycin Biosynthesis)