Regular paperDevelopment and validation of a capillary zone electrophoresis method for the determination of atropine, homatropine (cas 114173-36-3) and scopolamine in ophthalmic solutions

Add time:09/02/2019    Source:sciencedirect.com

A capillary zone electrophoresis method is described for the simultaneous determination of atropine, homatropine (cas 114173-36-3) and scopolamine. Successful results were obtained after optimization of the electrophoretic parameters such as buffer composition and pH. The best separation was achieved using a 100 mM Tris-phosphate running buffer at pH 7. The validation data proved that the method had the requisite selectively, reproducibility and linearity to be used for the assay of these compounds in pharmaceutical formulations. Dosage of the separate drugs in ophthalmic preparations is also presented.

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