Discrimination between primary necrosis and apoptosis by necrostatin-1 in ANNEXIN V (cas 136107-94-3)-positive/propidium iodide-negative cells

Add time:09/05/2019    Source:sciencedirect.com

Apoptotic cell death eventually results in secondary necrotic cell death, whereas caspase-independent primary necrotic cell death has been reported and its mechanism involving RIP1 and RIP3 has been recently elucidated. Dual staining with fluorescent Annexin V and propidium iodide (PI) has been used to discriminate apoptotic and necrotic cell death, in which Annexin V-positive/PI-negative staining is regarded as apoptosis and PI-positive staining as necrosis. Here we demonstrate that primary necrotic cells unexpectedly show Annexin V-positive/PI-negative staining before they become PI-positive, and that primary necrotic and apoptotic Annexin V-positive/PI-negative cells can be discriminated by necrostatin-1, an inhibitor of primary necrosis by inhibition of RIP1.

We also recommend Trading Suppliers and Manufacturers of ANNEXIN V (cas 136107-94-3). Pls Click Website Link as below: cas 136107-94-3 suppliers

Prev:Anti-ANNEXIN V (cas 136107-94-3) antibodies: are they prothrombotic?
Next:Imaging apoptosis in vivo using 124I-ANNEXIN V (cas 136107-94-3) and PET)