Regular articleSolution structure of the HIV-2 TAR-argininamide (cas 16709-23-2) complex1☆

Add time:09/09/2019    Source:sciencedirect.com

The trans-activating region (TAR) RNA-Tat protein interaction is important for activation of transciption in the human immunodeficiency virus (HIV). A model complex for this interaction composed of the two base bulge HIV-2 TAR and the amide derivative of arginine was studied by multidimensional heteronuclear NMR. Because of the improved spectral properties of the HIV-2 TAR complex, a larger number of NOEs in the bulge region were observed than in earlier studies of the HIV-1 TAR-argininamide complex. A total of 681 NOE distance restraints were collected and used to determine the solution structure of the HIV-2 TAR-argininamide complex. As observed in the previously proposed model from this lab, the two A-form stems co-axially stack and the critical U23 and the argininamide are located in the major groove. Model calculations including non-experimental restraints indicate that U23 is within hydrogen bonding distance to A27 consistent with the formation of a U·A·U base-triple. Base-triple formation helps open the major groove to increase the accessibility of G26 to hydrogen bond donors from the guanidinium group of argininamide. Argininamide binding is stabilized by stacking of the guanidinium group between the bases of A22 and U23, forming an argininamide sandwich.

We also recommend Trading Suppliers and Manufacturers of argininamide (cas 16709-23-2). Pls Click Website Link as below: cas 16709-23-2 suppliers

Prev:l-argininamide (cas 16709-23-2) improves the refolding more effectively than l-arginine
Next:NG-Acyl-argininamide (cas 16709-23-2)s as NPY Y1 receptor antagonists: Influence of structurally diverse acyl substituents on stability and affinity)