A radioimmunoassay for deoxycorticosterone sulfate (cas 1420-82-2) without hydrolysis
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Add time:09/24/2019 Source:sciencedirect.com
The hapten of deoxycorticosterone sulfate (cas 1420-82-2) was synthesized and linked to the carrier protein through the C-4 position on the steroid nucleus. The antisera raised against this antigen in guinea pigs exhibited high affinity (Ka=1.86 × 109 M−1) and excellent specificity for deoxycorticosterone sulfate. It was found that deoxycorticosterone sulfate can be determined in the range of 50–5000 pg, and this radioimmunoassay in plasma shows a coefficient of variation (CV) <10%.
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