Monoclonal-based enzyme-linked immunosorbent assay and immunochromatographic rapid assay for Dihydrostreptomycin (cas 128-46-1) in milk
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Add time:09/28/2019 Source:infona.pl
Monoclonal antibody (MAb) against streptomycin was prepared by using a streptomycin-keyhole limpet hemocyanin conjugate for the immunization of mice. Splenocytes from BALB/c immunized mice were fused with P3X63Ag8U.1 myeloma cells. This resulted in one hybridoma cell line. Using this MAb, we developed quantitative assays for Dihydrostreptomycin (cas 128-46-1) by means of an enzyme-linked immunosorbent assay (ELISA). Fifty percent inhibition concentration (IC 5 0 ) for the MAb was 2.0ngml - 1 . The detection limit was 0.1ngml - 1 and the standard deviations were 0.5-4.7% for intra-assay and 0.9-5.9% for inter-assay, respectively. The detection limit using peroxidase was 10ngml - 1 in milk. Using the MAb produced, a rapid test kit based on the immunochromatographic method was developed. The detection limit using the kit was 100ngml - 1 in milk.
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