Construction of gene expression system in hop (Humulus lupulus) lupulin gland using Valerophenone (cas 1009-14-9) synthase promoter

Add time:07/17/2019    Source:sciencedirect.com

SummaryThe promoter region of the Valerophenone (cas 1009-14-9) synthase (VPS) gene was isolated from hop (Humulus lupulus). VPS, a member of the chalcone synthase (CHS) super-family, catalyzes the biosynthesis reaction of the hop resin that significantly accumulates in the cone's secretory gland called the «lupulin gland». The typical H-box and G-box sequences, which exist in many plantsapos; CHS promoters and act as cis-elements for tissue specificity, UV-light induction, etc., were not found in the isolated VPS promoter, although the H-box-like sequence (CCTTACC, CCTAACC) and the core sequence (ACGT) of the G-box were observed. The transformation experiment using the VPS promoter-UIDA gene fusion revealed that the promoter acts not only in the lupulin gland but also in the glands of leaf and stem. On the other hand, the VPS promoter activity was not induced by UV-irradiation.

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