Mechanism driven structural elucidation of forced degradation products from hydrocortisone in solution

Add time:07/17/2019    Source:sciencedirect.com

Hydrocortisone degradation products 1, 2, 3, and 4 along with hemiacetal derivatives 5, 6, 7, and 8 were observed through stressed hydrocortisone in solution. Their structures were identified based on HPLC-UV, HPLC–MS, and HPLC-HRMS (high resolution/high accuracy mass spectrometry) analyses as well as reaction mechanistic investigation and synthesis for structural confirmation. 1 and 2 are a pair of E/Z isomers and they were generated through acid catalyzed tautomerization/dehydration of hydrocortisone. Incorporation of water to 1 and 2 resulted in the formation of 3. We also discovered new degradation product 4 which was converted from 3 by oxidation. The degradation products were synthesized by stressing hydrocortisone under the optimized conditions and their structures were characterized by NMR (1H/13C, COSY, HMBC, HSQC, NOESY) and HRMS analyses. The degradation pathway of hydrocortisone is postulated.

We also recommend Trading Suppliers and Manufacturers of Hydrocortisone IMpurity I (cas 103795-84-2). Pls Click Website Link as below: cas 103795-84-2 suppliers

Prev:Cationic lipid-conjugated hydrocortisone as selective antitumor agent
Next:Preparation of hydrocortisone nanosuspension through a bottom-up nanoprecipitation technique using microfluidic reactors)