Reference

Ascorbic acid in the pituitary gland

Ascorbic acid in the pituitary gland. 3. Bound ascorbic acid in the adenohypophysis. Kabrt, Jan (Med. Fac., Charles Univ., Prague, Czech.). Acta Univ. Carol., Med. 50-81-7 and 50-28-2 which are cas registry numbers are also used here., 28(5-6), 345-68 (English) 1982. CODEN: AUNCA9. ISSN: 0001-7116. DOCUMENT TYPE: Journal CA Section: 13 (Mammalian Biochemistry) Section cross-reference(s): 2 With the use of the batchwise method (gel filtration) or filtration on Diaflo ultrafilters, free and bound ascorbic acid in the rat adenohypophysis were sepd. In the adenohypophysis, 25-40% of the ascorbic acid was bound. In hypertrophic adenohypophyses of rats receiving estradiol, however, the ratio between free and bound ascorbic acid did not change. A smaller utilization of the binding capacity for ascorbic acid in adenohypophyses of the rats receiving estradiol is assumed. Problems of ascorbic acid action on adenohypophyseal functions are discussed and relations of ascorbic acid to the whole endocrine axis are outlined. .

Effect of varying amounts of ascorbate on collagen, elastin and lysyl oxidase synthesis in aortic smooth muscle cell cultures

Effect of varying amounts of ascorbate on collagen, elastin and lysyl oxidase synthesis in aortic smooth muscle cell cultures. Faris, Barbara; Ferrera, Rocco; Toselli, Paul; Nambu, Jiro; Gonnerman, Wayne A.; Franzblau, Carl (Sch. Med., Boston Univ., Boston, MA 02118, USA). Biochim. Biophys. Acta, 797(1), 71-5 (English) 1984. CODEN: BBACAQ. ISSN: 0006-3002. DOCUMENT TYPE: Journal CA Section: 13 (Mammalian Biochemistry) In the presence of ascorbate, there is an increase in collagen synthesis with a concomitant decrease in insol. elastin and lysyl oxidase activity in cultured rabbit aortic smooth muscle cells. 50-81-7 and 9059-25-0 are cas registry numbers of chemicals which are used as reagents here. Whereas the addn. of 0.5 mg ascorbate/mL medium enhances collagen synthesis and accumulation, detectable insol. elastin and lysyl oxidase activity remain essentially unchanged. However, at 2 mg ascorbate/mL, the integrity of the insol. elastin is lost and lysyl oxidase activity is decreased. Thus, by modifying the levels of ascorbate in the culture medium one can alter the nature of the extracellular matrix produced by the smooth muscle cells. .