Reference

Induction of somatic embryos in cotyledonary tissue of soybean, Glycine max L

Induction of somatic embryos in cotyledonary tissue of soybean, Glycine max L. Merr. Lippmann, Baerbel; Lippmann, Gunter (Inst. Zuechtungsforsch., Akad. Landwirtschaftswiss., Quedlinburg DDR-4300, Ger. Dem. Rep.). Plant Cell Rep., 3(6), 215-18 (English) 1984. CODEN: PCRPD8. ISSN: 0721-7714. DOCUMENT TYPE: Journal CA Section: 5 (Agrochemical Bioregulators) Section cross-reference(s): 11 A method for the induction of somatic embryos in soybean tissue cultures is described. Cotyledons from immature embryos were utilized as explant source. Supplementing the culture medium with auxins (2,4-D [94-75-7], MCPA [94-74-6], 2,4,5-T [93-76-5], NAA [86-87-3], IAA [87-51-4], IBA [133-32-4]) caused formation of meristematic tissue on cotyledon explants. The extent of meristematic tissue formed depended on the kind and concn. of auxin in the culture medium. With 2,4-D and MCPA, embryoids originated from meristematic tissue. Embryoid formation rates were influenced by the developmental stage of the embryos serving as explant source, and by the auxin concn. Addn. of cytokinins to the medium contg. 2,4-D or supplementing it with high sugar concns. inhibited the formation of meristematic tissue and of embryoids on cotyledon explants.

Rooting of carnation cuttings and effect of plant growth regulators on the root formation

Rooting of carnation cuttings and effect of plant growth regulators on the root formation. Ivanova, I.; Bumova, I. (Univ. Sofia, Sofia, Bulg.). God. Sofii. Univ. "Kliment Okhridski", Biol. Fak., Volume Date 1982, 72-75(4), 87-96 (Bulgarian) 1984. CODEN: GSUFD6. DOCUMENT TYPE: Journal CA Section: 5 (Agrochemical Bioregulators) The effect of different concns. of IAA [87-51-4], NAA [86-87-3], IBA [133-32-4], boric acid, and vitamin B1 [59-43-8] (sep. and in combination) on the rooting of 10 different varieties of carnation type Sim was studied. The base of cuttings was sunk in the powder before planting. The stimulation of root growth was best in 0.2% NAA. The addn. of 5% proline [147-85-3] increased the growth of roots. An addn. of 0.2% perozin [12122-67-7] was not inhibitory to the growth of roots. In the process of rooting the level of gibberellin-like substances increased. Stimulation of root growth is in direct correlation with the endogenous level of auxins and is in indirect correlation with the level of growth inhibitors and IAA oxidase [9027-85-4] activity.