3966
Y. Tabuchi et al. / Bioorg. Med. Chem. 17 (2009) 3959–3967
s, CH2OH), 5.12 (1H, t, J = 7.2 Hz, @CHCH2OH), 6.87 (1H, d,
J = 16.1 Hz, CH@CHC6H5), 7.10 (1H, dd, J = 7.3 and 1.5 Hz, 7-H),
7.27–7.34 (2H, m, 8-, 9-H), 7.39–7.47 (3H, m, 30-, 40-, 50-H),
7.58 (1H, d, J = 16.2 Hz, CH@CHC6H5), 7.75–7.77 (2H, m, 20-, 60-
H); m/z (EI) 347 (M+, 100), 330 (86), 303 (17), 244 (4), 217
(11), 131 (31), 103 (36).
Acknowledgments
This work was supported in part of a grant from the Ministry of
Education, Culture, Sports, Science and Technology for the ‘Univer-
sity–Industry Joint Research’ Project (2004–2008). We thank the
staff of the instrument analysis center of Mukogawa Women’s Uni-
versity for the NMR and MS measurements and elemental
analyses.
4. Evaluation of anti-bone resorption activity
Calvarial osteoblasts precultured to preconfluent from 1 to
2 day old ddY mice (Shizuoka Laboratory Animal Center, Hamama-
tsu, Japan) and fresh bone marrow cells from 5 week-old ddY male
mice (Shizuoka Laboratory Animal Center) were cocultured in
Supplementary data
Supplementary data (physical and spectral data of 5, 9 and 12)
associated with this article can be found, in the online version, at
a-MEM (pH 7.0)(Sigma Chemical Co., St Louis, MO, USA) containing
10% fetal calf serum (FCS, Moregate, Australia and New
Zealand),10 nM calcitriol (Wako Pure Chemical Ind., Osaka, Japan)
References and notes
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TM
5.3. AlamarBlue assay for cell cytotoxicity
TM
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TM
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