Enzymes in Oligosaccharide Synthesis: Active-Domain Overproduction, Specificity Study, and Synthetic Use of an α-1,2-Mannosyltransferase with Regeneration of GDP-Man

Article abstract of DOI:10.1021/jo00067a035

The catalytic domain of a membrane-bound α-1,2-mannosyltransferase (ManT) from yeast has been overexpressed in E.coli, at a level of approximately 0.7-0.8 units per L with a specific activity of about 1 U/mg (based on α-methylmannoside) after purification