IMPROVED METHODS FOR THE ENZYMIC PREPARATION AND CHROMATOGRAPHY OF OCTULOSE PHOSPHATES

Article abstract of DOI:10.1016/0008-6215(85)85050-3

High-yield methods are presented for the synthesis of D-glycero-D-altro-octulose 8-phosphate and 1,8-diphoshate, and D-glycero-ido-octulose 1,8-diphosphate and 8-phosphate.Formate ion-exchange and DEAE-Sephadex A-25 borate column chromatography were used for the isolation of these compounds.A new phenylboronate-containing solvent for p.c. and t.l.c. of sugar phosphates is described.The 13C-n.m.r. assignments of the major contributing structure of D-glycero-D-altro-octulose 8-phosphate in aqueous solution (pH 7.0) are presented.