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signal. Thus, the loading of CaTM-2 AM with a dispersing
agent is effective in producing a large fluorescence signal,
although it may have some influence on cellular homeostasis.
For the broader biological application of Ca2+ indicators
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its AM ester form are especially useful for neuronal Ca2+
imaging, but because of its localization in lysosomes in some
types of cultured cells, CaSiR-1 AM will sometimes be
unsuitable for the detailed analysis of signaling pathways
inside cells (see Figures S11–S15 in the Supporting Informa-
tion). Two other probes were also used successfully to monitor
cytoplasmic Ca2+; however, the AM ester forms of these
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AM ester loading method.
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applicable in GFP-expressing animals and cultured cells. We
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Received: December 25, 2012
Revised: January 26, 2013
Published online: February 25, 2013
Keywords: bioimaging · calcium · cell signaling · fluorescence ·
.
imaging agents
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ꢀ 2013 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
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