Synthesis of 5'-phosphonate linked thymidine deoxyoligonucleotides

Article abstract of DOI:10.1016/0040-4039(96)01437-2

5'-Deoxy-5'-phosphonothymidine was synthesized and coupled with 3'-deoxy-3'-hydroxy-methylthymidine to yield a 3'-C-O-P-5' linked thymidine dinucleotide. This dimer as its 3'-phosphoramidite was used to synthesize deoxyoligonucleotides.