Synthesis of fluorescent phosphatidylinositols using a novel inositol H- phosphonate

Article abstract of DOI:10.1016/S0040-4039(98)00418-3

Coupling of 1,2-diradyl-sn-glycerol 5 with the novel inositol H- phosphonate derivative, 6-O-benzyl-2,3:4,5-di-O-isopropylidene-myo-inositol H-phosphonate (3), gave fluorescent analogs of phosphatidylinositol (PtdIns, 1) and PtdIns(4,5)-bisphosphate (PtdIns(4,5)P₂, 2). Unlike the corresponding phosphoramidate, 3 was stable at -20 °C for several months, making it a useful intermediate for the synthesis of myo-inositol phospholipids.