Ultrafast cleavage and deprotection of oligonucleotides synthesis and use of C(Ac) derivatives

Article abstract of DOI:10.1080/07328319708006236

We have investigated the use of alkylamines as fast cleavage and deprotection reagents for the solid phase synthesis of oligonucleotides and found methylamine/ammonium hydroxide (or methylamine) as an efficient reagent. The transamination side product formed with the commonly used dC(b2) has been eliminated by the use of dC(Ac) phosphoramidite. This system has successfully been used in the synthesis of oligonucleotides and oligonucleoside phosphorothioates. DMT dC(Ac) hydrogen phosphonate and DMT ribo C(Ac)-2'-OMe phosphoramidite also have been prepared and used in the synthesis of oligonucleotides.

Full text of DOI:10.1080/07328319708006236

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Nucleosides and Nucleotides
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Ultrafast Cleavage and Deprotection of
Oligonucleotides Synthesis and Use of
CAc Derivatives
M. P. Reddy a , Naeem B. Hanna a & Firdous Farooqui a
a Beckman Instruments Inc. , 2500 Harbor Blvd., Fullerton, CA,
92834
Published online: 16 Aug 2006.
To cite this article: M. P. Reddy , Naeem B. Hanna & Firdous Farooqui (1997) Ultrafast Cleavage
and Deprotection of Oligonucleotides Synthesis and Use of CAc Derivatives, Nucleosides and
Nucleotides, 16:7-9, 1589-1598, DOI: 10.1080/07328319708006236
To link to this article: http://dx.doi.org/10.1080/07328319708006236
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