Tetrahedron Letters p. 3001 - 3004 (2001)
Update date:2022-09-26
Topics:
Hojo, Hironobu
Watabe, Jun
Nakahara, Yoshiaki
Nakahara, Yuko
Ito, Yukishige
Nabeshima, Kazuki
Toole, Bryan P.
The extracellular Ig domain I of Emmprin (34-94) carrying a chitobiose unit at Asn44 was chemically synthesized. Boc-Asn with a benzyl-protected chitobiose unit was synthesized and used for the preparation of peptide thioester with the sequence of Emmprin (34-58) by Boc strategy. C-Terminal peptide amide (59-94) was also prepared by the solid-phase method. These segments were condensed by activation of the thioester group by silver ions to obtain a protected form of Emmprin (34-94)-NH2. After deprotection and air oxidation, the desired Emmprin (34-94)-NH2 with chitobiose was successfully obtained.
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