Serine-Protease-Assisted Synthesis of Peptide Substrates for α-Chymotripsin

Article abstract of DOI:10.1002/hlca.19820650605

δ-Chymotrypsin catalyzes peptide-bond formation between acylated amino-acid and peptide esters as the carboxyl component and amino-acid and peptide amides as the amino component.The conditions under which enzyme-catalyzed coupling can be used for fragment condensation in peptide synthesis is investigated.To illustrate the method the synthesis of tetra-, penta and hexapeptides of the structure Ac-L_xn-...-L_xl-L_yl-...-L_ym-NH2 with L_xl = Tyr, designed as substrates for α-chymotrypsin is described.