RACEMIZATION OF PHENYLGLYCINE CATALYZED BY PYRIDOXAL. CATALYTIC EFFECT OF PHOSPHATE ION

Article abstract of DOI:10.1016/S0040-4039(00)96816-3

The racemization reaction of phenylglycine catalyzed by pyridoxal was investigated.The reaction was found to be catalyzed by the phosphate buffer and the detailed kinetic analysis demonstrated that two catalytic terms, k_A2-> and K_AB2->3-> were implied in the present racemization of phenylglycine.The values of k_A and k_AB were determined from the dependency of the observed rates on the concentrations of HPO4^2- and PO4^3- to be 1.5E-3 s^-1 M^-1 and 3.1E-1 s^-1 M^-2, respectively.The latter is the first example of the third order catalytic term in the pyridoxal dependent amino acid racemization.