Vol. 25, No. 13 (2013)
Antibacterial and Antifungal Activities of Bisbenzoylthiourea Compounds 7553
TABLE-3
RESULTS OF ANTIMICROBIAL ACTIVITY OF SYNTHESIZED COMPOUNDS 3a-3e
Diameter of zone of inhibition (mm)
Corynebacterium Staphylococcus
Gibberella
Compound
Pseudomonas
aeruginosa
Escherichia
coli
Pythium
Cochliobolus
sativus
diphtheriae
6.8 0.25
4.3 0.2
7.7 0.15
15.1 0.5
11.2 0.5
13.7 0.4
–
aureus
5.9 0.2
5.6 0.15
6.2 0.2
12.1 0.3
8.6 0.4
sp.
aphanidermatum
3.1 0.1
–
5.4 0.15
–
6.9 0.25
11.9 0.45
8.9 0.3
8.1 0.3
7.9 0.3
8.8 0.2
17.6 0.7
13.1 0.4
–
6.3 0.2
–
9.3 0.4
16.8 0.5
15.6 0.3
–
8.2 0.1
8.4 0.35
8.9 0.1
17.5 0.6
14.3 0.6
–
3a
3b
5.4 0.15
14.8 0.6
10.4 0.35
16 0.25
–
3c
3d
3e
Ampicillin
Nystatin
15.9
0.7
13.6
0.5
–
–
15.4 0.3
18.1 0.6
17.3 0.5
–: Indicates no activity.
and sabourd's medium solution was poured into sterilized
petridishes and allowed to attain room temperature. Seed layer
medium which contains the previously grown subculture was
lawned into the Petri dishes. Cups were made using sterile
borer of 5 mm diameter. To these cups 0.5 mL of the drug
solution (50 µg/mL), standard solution were added and allowed
to cool for 1 h to facilitate diffusion. The plate was incubated
at 37 ºC for 48 h. Zone of inhibition around wells were measured.
All tests were repeated three times to confirm the results. The
results are showed in Table-3.
standard drugs, but the antifungal activity is more prominent.
So the bright prospects of the compounds synthesized above
in against agricultural disease fungal and the potential of anti-
microbial have been brought out.
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A series of bisbenzoylthiourea compounds 3a-3e have
been synthesized with good yields and the structures are
confirmed by elemental analyses, IR spectra and 1H NMR data.
All the synthesized compounds were tested for their anti-
microbial activities and 7 species of microbial have been
choosen. Because two classes of the compounds synthesized
above (3d and 3e) were oxime compounds and they were
reported as oxygen eliminating agent, so they were tested by
two obligate aerobes bacteria and two facultative aerobic
bacteria to prove their oxygen eliminating activities.Acylthio-
urea compounds also reported varied biological activities
especially in the aspect of agricultural disease prevention and
treatment, so they were tested by 3 species of commom agricul-
tural disease fungal to prove their antifungal activities. The
results presented: compound 3d showed the highest antibac-
terial and antifungal activities and close to the standard drug.
For some bacteria and fungal, compound 3d even have higher
antimicrobial activities than the standard drugs like the
experiment of Corynebacterium diphtheria, Gibberella sp and
Cochliobolus sativus. Compounds 3e and 3c also possess
higher antibacterial and antifungal activities. Compounds 3d
and 3e were shown higher inhibition on the two Obligate
aerobes bacteria and lower inhibition on the two facultative
aerobic bacteria because of their oxygen eliminating activities.
At the concentration of 50 µg/mL, compound 3b have no
inhibition on gram-negative bacteria like the experiment of
Pseudomonas aeruginosa and Escherichia coli, it possess the
lowest antibacterial and antifungal activities as compared to
the other compounds. In a word, the antimicrobial experiments
had indicated most of the synthesized compounds have shown
good antibacterial and antifungal activities as compared to their
Chim. Acta, 362, 1129 (2009).