N,N-BISPHOSPHORODIAMIDIC CHLORIDE: A NOVEL COUPLING REAGENT IN THE SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES.

Article abstract of DOI:10.1016/S0040-4039(00)98833-6

Use of a novel coupling reagent, N,N-bis<2-oxo-3-oxazolidinyl>phosphorodiamidic chloride (BOPDC) in the synthesis of oligodeoxyribonucleotides is described.This reagent allows the synthesis of phosphotriesters in high yields (70-80 percent) without detectable side reactions.Synthesis of a hexanucleotide, d(A-A-C-C-C-G) is presented as an example.