Tetrahedron p. 1391 - 1398 (1999)
Update date:2022-08-03
Topics:
Bianchini, Ercolina
Chinaglia, Nicola
Dean, Mariangela
Giovannini, Pier Paolo
Medici, Alessandro
Pedrini, Paola
Poli, Silvia
The preparative-scale regio- and stereo-specific oxidation of hydroxy groups and reduction of keto functions at C(3) of several C21 bile acids, catalyzed by a new 3α-hydroxysteroid dehydrogenase (3α-HSDH) is reported. The crude enzyme, isolated from the cells of Pseudomonas paucimobilis, revealed the presence of a further enzymatic fraction containing a secondary alcohol dehydrogenase (SADH), that has been used to recycle the cofactor.
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