POLYMER SUPPORTED DNA SYNTHESIS USING HYDROXYBENZOTRIAZOLE ACTIVATED PHOSPHOTRIESTER INTERMEDIATES

Article abstract of DOI:10.1016/S0040-4039(00)88244-1

In the present method appropriate N-protected-5'-O-(4,4'-dimethoxytrityl)-2'-deoxynucleosides are phosphorylated with the reagent 2-chlorophenyl-O,O-bis(1-benzotriazolyl)-phosphate and the 3'-phosphorylated intermediates thus obtained are used directly to