Mode of action of (1→4)-β-D-arabinoxylan arabinofuranohydrolase (AXH) and α-L-arabinofuranosidases on alkali-extractable wheat-flour arabinoxylan

Article abstract of DOI:10.1016/0008-6215(93)84099-R

Arabinoxylan-derived oligosaccharides were treated with (1→4)-β-D-arabinoxylan arabinofuranohydrolase (AXH) and two types of α-L-arabinofuranosidase, A and B. Analysis of reaction products by high performance anion-exchange chromatography indicated the removal of arabinofuranosyl groups from singly substituted xylopyranosyl residues. In addition, differences in the specificity of these enzymes towards the various differently substituted oligosaccharides were observed. ¹H NMR spectroscopy and methylation analysis of alkali-extractable wheat-flour arabinoxylan treated with AXH confirmed the specificity of AXH towards (1→3)-linked arabinofuranosyl groups on singly substituted xylopyranosyl residues. With these techniques, α-L-arabinofuranosidase B was found to cause minor changes in (1→2)- and (1→3)-linked arabinofuranosyl groups on doubly substituted xylopyranosyl residues. Arabinoxylan-derived oligosaccharides were treated with (1 → 4)-B-D-arabinoxylan arabinofuranohydrolase (AXH) and two types of A-L-arabinofuranosidase, A and B. Analysis of reaction products by high performance anion-exchange chromatography indicated the removal of arabinofuranosyl groups from singly substituted xylopyranosyl residues. In addition, differences in the specificity of these enzymes towards the various differently substituted oligosaccharides were observed. ¹H NMR spectroscopy and methylation analysis of alkali-extractable wheat-flour arabinoxylan treated with AXH confirmed the specificity of AXH towards (1 → 3)-linked arabinofuranosyl groups on singly substituted xylopyranosyl residues. With these techniques, A-L-arabinofuranosidase B was found to cause minor changes in (1 → 2) and (1 → 3) linked arabinofuranosyl groups on doubly substituted xylopyranosyl residues.