Stereoselective reduction of ethyl 4-chloro-3-oxobutanoate by fungi

Article abstract of DOI:10.1271/bbb.65.1676

The enantioselectivity of ECAA to ECHB by eight fungi of four genus was evaluated. All strains showed (S)-selectivity, and Cylindrocarpon sclerotigenum IFO 31855 gave the highest yield and good optical purity (e.e.; >99%). Cell-free extract and acetone-dried cells of C. sclerotigenum IFO 31855 reduced ECAA to (S)-ECHB in the presence of NADPH (e.e.; > 99%) and the e.e. was not decreased by heat treatment of the cell-free extract or the acetone-dried cells. The active fractions shown by two peaks on a DEAE-Toyopearl 650 M column gave preferentially (S)-ECHB (e.e.;>99%).

Full text of DOI:10.1271/bbb.65.1676

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Stereoselective Reduction of Ethyl 4-chloro-3-
oxobutanoate by Fungi
Yuri SARATANIa, Eiji UHEDAa, Hiroaki YAMAMOTOb, Atsuo NISHIMURAa & Fumiki
YOSHIZAKOa
a Research Institute for Advanced Science and Technology, Osaka Prefecture University
b Tsukuba Research Center, Daicel Chemical Industries Ltd.
Published online: 22 May 2014.
To cite this article: Yuri SARATANI, Eiji UHEDA, Hiroaki YAMAMOTO, Atsuo NISHIMURA & Fumiki YOSHIZAKO (2001)
Stereoselective Reduction of Ethyl 4-chloro-3-oxobutanoate by Fungi, Bioscience, Biotechnology, and Biochemistry, 65:7,
1676-1679, DOI: 10.1271/bbb.65.1676
To link to this article: http://dx.doi.org/10.1271/bbb.65.1676
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