ChemCatChem
10.1002/cctc.201801050
FULL PAPER
Isolation protocols
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5
0WX8 cation exchange column. First, the resin was conditioned by
washing with aqueous NH ( 2 M, 2×30 ml), HCl (2 M, 2×30 ml) and H
4×30 ml). Second, the crude reaction mixture was acidified with HCl (1 M)
and loaded onto the column. Finally, the column was washed with HCl (1
M, 2×30 ml), H O (4×30 ml) and eluted with NH (2 M, 4 ×30 ml). Fractions
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2
O
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[
[
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solvent was removed by evaporation, and the product was dried overnight
under vacuum. The so obtained solid was washed with (EtOH/H O, 9:1)
2
1
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This work was financially supported by the National First-class
Discipline Program of Light Industry Technology and Engineering
(
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Keywords: β-alanine • enzyme cascade • L-aspartase • L-
aspartate-α-decarboxylase • cooperativity
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