Collection of Czechoslovak Chemical Communications p. 731 - 741 (2010)
Update date:2022-08-10
Topics:
Rodrigues, Jose Antonio
Valente, Ines Maria
Goncalves, Luis Moreira
Pacheco, Joao Grosso
Barros, Aquiles Araujo
A differential pulse polarographic (DPP) method has been developed for the determination of ascorbic acid (AA) and dehydroascorbic acid (DHA), the two main forms of Vitamin C. The method consists of the DPP analysis of a quinoxaline obtained by the derivatization of DHA with o-phenylenediamine. Results using the proposed method correlated well with those obtained by two reference methodologies: the common iodometric method and a published chromatographic methodology. It was also used in the study of Vitamin C degradation in fruit juices, showing that it involves an initial oxidation of AA to DHA, followed by ydrolytic degradation of the latter.
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