Bioscience, Biotechnology and Biochemistry p. 2212 - 2215 (1999)
Update date:2022-08-17
Topics:
Shiraiwa, Tadashi
Kubo, Motoki
Fukuda, Keiji
Kurokawa, Hidemoto
DL-Threonine (DL-Thr) was optically resolved by replacing crystallization with L-alanine (L-Ala) as an optically active cosolute. D-Thr was preferentially crystallized from a supersaturated aqueous solution of DL-Thr in the presence of L-Ala. Optical resolution was successfully achieved to afford D-Thr with an optical purity of 96-98% and L-Thr of 91-95%. The partially resolved D- and L-Thr were recrystallized from water, taking account of the solubility of DL-Thr, to efficiently yield both enantiomers in an optically pure form.
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