Interaction of Poly and Its Derivatives with Lipid Membrane

Article abstract of DOI:10.1246/bcsj.61.3983

Poly (PolyNAPGly, 5) and its derivatives were synthesized as a model peptides of the membrane-bound enzymes, and investigated on their interactions with lipid membrane.PolyNAPGly (5) was shown to be distributed to dipalmitoylphosphatidyl choline biolayer membrane without destruction of the vesicle structure.Dodecyl groups were introduced to the side chains of PolyNAPGly (5), and found to facilitate binding of the polymer to lipid membrane.However, the introduction of the excess amount of hydrophobic groups to PolyNAPGly (5) caused the formation of polymer micelles, and the addition of such polymers to lipid vesicles destroyed the membrane structure.The derivative of PolyNAPGly (5) (n=35) bearing one or two dodecyl groups and some N'-benzyloxycarbonylhistidyl groups was found to be bound tightly to lipid membrane without destruction of the membrane structure.The addition of these polypeptides to vesicles decreased the membrane fluidity.