Synthesis and preliminary binding studies of 4,4-ditritio(-)nicotine of high specific activity

Article abstract of DOI:10.1021/jm00182a028

4,4-Ditritio-(-)nicotine (5) of high specific activity (4.7 Ci/mmol) has been synthesized from (-) nicotine via the readily prepared 4,4-dibromocotinine. Scatchard analysis of the binding of 5 to the crude mitochondrial fraction of whole rat brain revealed a K(a) of 4.7 x 10⁶ M^-1 and 13 fmol of binding sites/mg of protein.