Analytical Techniques for the Determination of Chemical Exchange Rate Constants with Application to the Creatine Kinase Reaction

Article abstract of DOI:10.1016/0022-2364(90)90003-R

A method for determining biochemical exchange rates for analysis of inversion recovery experiments is described.As used in the current application, the technique involves fitting multiexponential equations to the magnetization recovery curves for PCr and ATP.From the parameters of the exponential fits expressions for the forward and reverse rate constants are derived.Results obtained with this technique were compared with inversion transfer and with previously published results using saturation transfer.The addition of a third exchanging species was also investigated and found to have no significant effect on the calculated values for the forward and reverse rate constants.In addition, the feasibility of using an abbreviated 6-point sampling strategy was evaluated; values for the rate constants were similar to those obtained using all 21 data points.The results of this study indicate that chemical exchange rate constants can be determined using inversion recovery techniques which avoid many of the difficulties associated with selective excitation methods.