Isolation and investigation of the ethylene-producing enzyme of cotton

Article abstract of DOI:10.1007/BF02323297

The activity of the ethylene-producing enzyme (EPE) in a homogenate of cotton leaves and in fractions obtained via differential centrifugation and fractional precipitation is studied. A model system is developed for testing EPE. The effect of known activators and inhibitors of ethylene biosynthesis on the EPE activity is studied. Chromatographic methods are used to prepare the highly purified enzyme from the water-soluble fraction of cotton proteins and to identify it as ACC-oxidase. The enzyme has MM ～38 kDa and converts aminocyclopropanecarboxylic acid (ACC) into ethylene.