Chemistry - An Asian Journal p. 276 - 281 (2013)
Update date:2022-08-11
Topics:
Chen, Jian
Jiao, Huping
Li, Wenying
Liao, Dongli
Zhou, Huipeng
Yu, Cong
A tetracationic perylene probe (probe 1) was designed and synthesized. Probe 1 was used for the real-time fluorescence turn-on assay of alkaline phosphatase (ALP) activity and inhibitor screening. Probe 1 monomer fluorescence could be very efficiently quenched by ATP through the formation of an ATP/probe 1 complex. ALP triggered the degradation of ATP, the breakdown of the ATP/probe 1 complex, and the recovery of the probe 1 monomer fluorescence. In the presence of an ALP inhibitor, a decrease in fluorescence recovery was observed. Copyright
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