Scale-up and Production

NMR spectra Characterization Melting point HPLC Optimization HCl salt Potassium phosphate and potassium carbonate Pyrrole Benzoate Scheme 7 Karl Fischer titration Round-bottomed flask Protecting groups Biological testing Purification and characterization Biological Assays Structure-Activity Relationship (SAR) Studies Clinical Trials Medicinal Chemistry Optimization Purification and Isolation In Vivo Studies Regulatory Approval Market Launch Design and Synthesis Protection and Deprotection Lead Compound Identification Pharmacokinetic and Toxicological Studies Further Optimization Biochemical Assays Pharmacokinetics and Pharmacodynamics Rational Design Synthesis of 5-Fluorouracil Protection of the N1 Amine Synthesis of the 1,4-Oxaheterocyclic Moiety Attachment of the 1,4-Oxaheterocyclic Moiety to 5-Fluorouracil Target Identification and Validation Chemical Synthesis of the N-Acetylneuraminic Acid Precursor Enzymatic N-5 Modification Selection of N-5 Substituent Enzymatic Reaction Conditions Intellectual Property Genetic Engineering Fermentation In Vivo Efficacy Studies Core Structure Collaboration and Research Analytical Methods Toxicology Studies Synthesis of Methanocarbaribo Uracil Core Nucleoside Attachment